1-Step Polymorphs, Human Cell Separation
To Order Contact us: lieven@wlsolutions.be
1.Preparation of the human blood samples.
For the best results, use whole blood treated with an anticoagulant
such as EDTA, heparin or citrate. We recommend EDTA (K salt) for
the best result. The blood should be used within two hours of
drawing from the donor. The blood samples and the
Polymorphprep™ solution should be at a temperature of 18-22 °C
and during centrifugation also kept within these limits.
2. Preparation of gradient
Layer 5.0 ml of anti-coagulated whole blood over 5.0 ml of
Polymorphprep™ in a 15 ml centrifuge tube or 18 ml of blood over
18 ml Polymorphprep™ in a 50 ml tube. Take care to avoid mixing
of the blood with the separation fluid. Always use equal volumes of
blood and PolymorphprepTM and select a tube that maintains a
similar geometry of layers to the recommended 5 ml + 5 ml in a 15
ml tube.
3. Separation procedure
Centrifuge the tubes at 500-550 gav for 30 minutes in a swing-out
rotor at 18-22 °C. Allow the rotor to decelerate without the break.
Centrifugation for longer times or higher centrifugal force will result
in the PMNs migrating further down towards the pelleted
erythrocytes.
4. Unloading the gradient
After centrifugation, two leukocyte bands should be visible. The top
band at the sample/medium interface will consist of mononuclear
cells and the lower band of PMNs; the erythrocytes are pelleted.
The cell bands are best harvested using a 2 ml syringe attached to
a flat-tipped metal filling cannula (0.8 mm inner diameter). The
PMNs should be diluted by addition of one volume of 0.45% NaCl
solution or culture medium at 0.5 normal concentration in order to
restore normal osmolality. For further dilutions use normal saline or
culture medium.
5. Washing
The suspension is transferred to a 15 ml tube and the cells pelleted
at approx. 400 g for 10 min (18-22 °C). They are resuspended in
saline or culture medium, sedimented again and then resuspended
in a medium compatible with the subsequent analysis.
Cytosol/Particulate Separation Kit |
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| 55R-1356 | 50 assays | EUR 661.2 |
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Description: Cytosol/Particulate Separation Kit for use in the research laboratory |
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Cytosol/Particulate Separation Kit |
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| K2112-50 | 50 assays | EUR 501.6 |
Cytosol/Particulate Separation Kit |
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| K267-50 | EUR 483.6 | |
Techne Separation Membranes 200x200mm |
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| OVE3038 | PK5 | EUR 142.5 |
Human Genomic DNA |
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| X11000 |
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Human Brain Genomic DNA |
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| X11001 |
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100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM) |
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| 25-072-CI | 100 mL/pk | EUR 63.6 |
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Description: Media Catalog; Cell Culture Reagents |
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Corning 500mL LSM (Lymphocyte Separation Medium) |
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| 25-072-CV | EACH | EUR 135.66 |
WHATMAN(R) TG100 SEPARATION GAUZE DIAM |
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| WHA10423029 | EACH | EUR 145.92 |
OrgFrontier? Viable/Non-Viable Cells Separation Kit |
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| K850-10 | EUR 652.8 | |
SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag |
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| E80025 |
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Methylamp One-Step DNA Modification Kit |
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| P-1010-1 | 40 Samples | EUR 148.5 |
Histone H3 Methylation Antibody Panel Pack I – Active Genes |
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| C10000 |
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Histone H3 Methylation Antibody Panel Pack I – Repression Genes |
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| C10001 |
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Histone H3 Methylation Antibody Panel Pack II – Active Genes |
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| C10002 |
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Histone H3 Methylation Antibody Panel Pack II – Repression Genes |
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| C10003 |
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Histone H3 Methylation Antibody Panel Pack III – Active Genes |
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| C10004 |
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Histone H3K4 Methylation Antibody Panel Pack |
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| C10005 |
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Histone H3K9 Methylation Antibody Panel Pack |
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| C10006 |
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Histone H3K27 Methylation Antibody Panel Pack |
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| C10007 |
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Histone H3K36 Methylation Antibody Panel Pack |
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| C10008 |
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Histone H3K79 Methylation Antibody Panel Pack |
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| C10009 |
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Histone H3 Acetylation Antibody Panel Pack I |
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| C10010 |
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Histone H3 Acetylation Antibody Panel Pack II |
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| C10011 |
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Histone H4K20 Methylation Antibody Panel Pack |
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| C10012 |
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Histone H4 Acetylation Antibody Panel Pack |
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| C10013 |
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Histone H3 Phosphorylation Antibody Panel Pack |
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| C10014 |
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Histone H3R2 Methylation Antibody Panel Pack |
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| C10015 |
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Histone H3R8 Methylation Antibody Panel Pack |
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| C10016 |
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Histone H3R17 Methylation Antibody Panel Pack |
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| C10017 |
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Histone H3R26 Methylation Antibody Panel Pack |
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| C10018 |
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Histone H4R3 Methylation Antibody Panel Pack |
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| C10019 |
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DNA Methylation Antibody Panel Pack I |
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| C20000 |
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DNMT3A Protein |
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| E11000 |
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TET1 Protein (Active) |
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| E12002 |
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DNMT3B Protein |
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| E14000 |
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DNMT1 Protein |
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| E15000 |
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HDAC1 Protein |
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| E24002 |
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HDAC10 Protein |
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| E24003 |
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HDAC11 Protein |
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| E24004 |
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HDAC2 Protein |
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| E24005 |
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HDAC3 Protein |
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| E24006 |
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HDAC4 Protein |
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| E24007 |
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HDAC5 Protein |
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| E24008 |
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HDAC6 Protein |
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| E24009 |
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HDAC7 Protein |
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| E24010 |
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HDAC8 Protein |
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| E24011 |
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HDAC9 Protein |
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| E24012 |
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H3F3A Protein |
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| E35003 |
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H4 Protein |
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| E35005 |
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SOD1 Protein |
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| E70000 |
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Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated |
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| A12001 |
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Goat Anti-Rat Secondary Antibody, Biotin Conjugated |
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| A12002 |
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HRP-Goat Anti-Mouse Secondary Antibody |
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| A12003 |
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HRP-Goat Anti-Rabbit Secondary Antibody |
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| A12004 |
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EpiMag HT (96-Well) Magnetic Separator |
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| Q10002 |
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EpiMag 96-Well Microplate (5/pack) |
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| Q10003 |
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HIF-1 Alpha Cell Based ELISA Kit |
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| CBA-281 | 96 assays | EUR 734.4 |
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Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat. |
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ACE2, His-Tag Protein |
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| E80019 |
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SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag |
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| E80020 |
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SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag |
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| E80021 |
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SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag |
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| E80022 |
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SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag |
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| E80023 |
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SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag |
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| E80024 |
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SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion |
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| E80026 |
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SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag |
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| E80027 |
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Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial |
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| E80028 |
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Stacking platform large 12x12 w flat mat (3.0 separation) |
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| MIX7114 | EACH | EUR 150.48 |
Stacking platform large 12x12 w dimpled mat (3.0 separation) |
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| MIX7116 | EACH | EUR 155.04 |
Rac1 Activation Assay |
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| STA-401-1 | 20 assays | EUR 908.4 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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Rap1 Activation Assay Kit |
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| STA-406-1 | 20 assays | EUR 908.4 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Arf1 Activation Assay |
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| STA-407-1 | 20 assays | EUR 908.4 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Human LOX-1 ELISA Kit |
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| STA-387 | 96 assays | EUR 936 |
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Description: Cell Biolabs? Human LOX-1 ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human LOX-1 in plasma, serum or cell culture supernatant samples. The kit has a detection sensitivity limit of 40 pg/mL human LOX-1. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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VCAM1 Human, Vascular cell adhesion molecule 1 Human Recombinant Protein, HEK |
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| PROTP19320-1 | Regular: 50ug | EUR 380.4 |
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Description: VCAM1 Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 682 amino acids (25-698). VCAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. |
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ESM1 Human, Endothelial Cell-Specific Molecule 1 Human Recombinant Protein, HEK |
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| PROTQ9NQ30-1 | Regular: 10ug | EUR 380.4 |
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Description: ESM1 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 20-184) containing 175 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 46.0kDa (calculated). |
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STEP antibody |
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| 10R-8556 | 100 ul | EUR 471.6 |
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Description: Mouse monoclonal STEP antibody |
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Additional platform separators (4 ea.). Adds 1.25" to platform separation |
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| BR2000-SP | 1 PC | EUR 111.94 |
Addi platform separators (4 ea) Add 1.25 to platform separation |
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| MIX7118 | EACH | EUR 60.42 |
Methylamp 96 DNA Modification Kit |
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| P-1008 |
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EpiQuik Nuclear Extraction Kit |
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| OP-0002 |
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Nucleic Acid Isolation Enhancer (Glycogen Solution) |
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| R-1001 |
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Methylamp PCR Enhancer |
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| R-1002 |
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Streptavidin: HRP Conjugate |
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| R-1098 |
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Streptavidin |
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| R-1100 |
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Protease Inhibitor Cocktail |
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| R-1101 |
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Streptavidin-Coated Strip Microwell Plate (Flat) |
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| R-1102 |
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DNA High Binding Solution |
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| R-1103 |
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DTT Solution |
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| R-1104 |
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PDCD1LG2 Human, Programmed Cell Death 1 Ligand 2 Human Recombinant Protein, Sf9 |
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| PROTQ9BQ51-1 | Regular: 10ug | EUR 380.4 |
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Description: PDCD1LG2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 423 amino acids (20-200a.a.) and having a molecular mass of 47.7kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).;PDCD1LG2 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques. |
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Human Alpha 1 Antitrypsin ELISA Kit |
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| PRB-5034 | 96 assays | EUR 686.4 |
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Description: Our Human Alpha 1 Antitrypsin ELISA Kit is a sandwich ELISA for the detection of human alpha 1 antitrypsin in plasma, serum, lysates, and other biological fluids. Samples or standards are added to an anti-A1AT antibody coated plate followed by the addition of a biotinylated anti-human A1AT antibody. A streptavidin enzyme conjugate is then added for detection. The absorbance is read at 450nm and the level of A1AT in samples is determined based on the provided human A1AT standard curve. |
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CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit |
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| OP-0005 |
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Rac1 Activation Assay Kit, Trial Size |
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| STA-401-1-T | 5 assays | EUR 483.6 |
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit. |
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Rap1 Activation Assay Kit, Trial Size |
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| STA-406-1-T | 5 assays | EUR 483.6 |
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit. |
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Arf1 Activation Assay Kit, Trial Size |
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| STA-407-1-T | 5 assays | EUR 483.6 |
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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit. |
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Human Alpha 1 Acid Glycoprotein ELISA Kit |
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| PRB-5044 | 96 assays | EUR 686.4 |
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Description: Our Human ?1AG ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human ?1AG in plasma, serum, urine, cell or tissue lysate samples. The kit has a detection sensitivity limit of 30 pg/mL human ?1AG. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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AmpliStain anti Mouse 1 Step (HRP) |
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| 75R-1061 | 6 ml | EUR 363.6 |
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Description: Mouse antigen staining reagent for use in IHC |
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AmpliStain anti Rabbit 1 Step (HRP) |
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| 75R-1062 | 6 ml | EUR 363.6 |
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Description: Rabbit antigen staining reagent for use in IHC |
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AmpliStain anti Goat 1 Step (HRP) |
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| 75R-1064 | 6 ml | EUR 429.6 |
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Description: Goat antigen staining reagent for use in IHC |
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H. Pylori Rapid Stain (1-Step) |
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| HPS030 | 30 ml | EUR 75.6 |
H. Pylori Rapid Stain (1-Step) |
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| HPS500 | 500 ml | EUR 121.2 |
H. Pylori Rapid Stain (1-Step) |
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| HPS999 | 1000 ml | EUR 156 |
amfiRivert 1-Step RT-PCR Kit |
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| R5003-050 | 50 rxns | EUR 262.8 |
amfiRivert 1-Step RT-PCR Kit |
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| R5003-100 | 100 rxns | EUR 400.8 |
amfiRivert 1-Step RT-PCR Kit |
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| R5003-200 | 200 rxns | EUR 562.8 |
1-Step Polymorphs, Human Cell Separation