1-Step Polymorphs, Human Cell Separation

1-Step Polymorphs, Human Cell Separation 

To Order Contact us: lieven@wlsolutions.be

 

Polymorphprep density Medium
Polymorphprep density Medium

1.Preparation of the human blood samples.
For the best results, use whole blood treated with an anticoagulant
such as EDTA, heparin or citrate. We recommend EDTA (K salt) for
the best result. The blood should be used within two hours of
drawing from the donor. The blood samples and the
Polymorphprep™ solution should be at a temperature of 18-22 °C
and during centrifugation also kept within these limits.

2. Preparation of gradient
Layer 5.0 ml of anti-coagulated whole blood over 5.0 ml of
Polymorphprep™ in a 15 ml centrifuge tube or 18 ml of blood over
18 ml Polymorphprep™ in a 50 ml tube. Take care to avoid mixing
of the blood with the separation fluid. Always use equal volumes of
blood and PolymorphprepTM and select a tube that maintains a
similar geometry of layers to the recommended 5 ml + 5 ml in a 15
ml tube.

3. Separation procedure
Centrifuge the tubes at 500-550 gav for 30 minutes in a swing-out
rotor at 18-22 °C. Allow the rotor to decelerate without the break.
Centrifugation for longer times or higher centrifugal force will result
in the PMNs migrating further down towards the pelleted
erythrocytes.

4. Unloading the gradient
After centrifugation, two leukocyte bands should be visible. The top
band at the sample/medium interface will consist of mononuclear
cells and the lower band of PMNs; the erythrocytes are pelleted.
The cell bands are best harvested using a 2 ml syringe attached to
a flat-tipped metal filling cannula (0.8 mm inner diameter). The
PMNs should be diluted by addition of one volume of 0.45% NaCl
solution or culture medium at 0.5 normal concentration in order to
restore normal osmolality. For further dilutions use normal saline or
culture medium.

5. Washing
The suspension is transferred to a 15 ml tube and the cells pelleted
at approx. 400 g for 10 min (18-22 °C). They are resuspended in
saline or culture medium, sedimented again and then resuspended
in a medium compatible with the subsequent analysis.

Polymorphprep PMN
Polymorphprep PMN

Density Gradient information

Axis Shield Polymorphprep PMN
Axis Shield Polymorphprep PMN

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 661.2
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

K2112-50 50 assays
EUR 501.6

Cytosol/Particulate Separation Kit

K267-50
EUR 483.6

Techne Separation Membranes 200x200mm

OVE3038 PK5
EUR 142.5

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Corning 500mL LSM (Lymphocyte Separation Medium)

25-072-CV EACH
EUR 135.66

WHATMAN(R) TG100 SEPARATION GAUZE DIAM

WHA10423029 EACH
EUR 145.92

OrgFrontier? Viable/Non-Viable Cells Separation Kit

K850-10
EUR 652.8

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

Methylamp One-Step DNA Modification Kit 

P-1010-1 40 Samples
EUR 148.5

Histone H3 Methylation Antibody Panel Pack I – Active Genes

C10000
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack I – Repression Genes

C10001
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Active Genes

C10002
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Repression Genes

C10003
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack III – Active Genes

C10004
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3K4 Methylation Antibody Panel Pack

C10005
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K9 Methylation Antibody Panel Pack

C10006
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K27 Methylation Antibody Panel Pack

C10007
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K36 Methylation Antibody Panel Pack

C10008
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K79 Methylation Antibody Panel Pack

C10009
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Acetylation Antibody Panel Pack I

C10010
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Acetylation Antibody Panel Pack II

C10011
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H4K20 Methylation Antibody Panel Pack

C10012
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H4 Acetylation Antibody Panel Pack

C10013
  • EUR 754.26
  • EUR 470.80
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Phosphorylation Antibody Panel Pack

C10014
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R2 Methylation Antibody Panel Pack

C10015
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R8 Methylation Antibody Panel Pack

C10016
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R17 Methylation Antibody Panel Pack

C10017
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R26 Methylation Antibody Panel Pack

C10018
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H4R3 Methylation Antibody Panel Pack

C10019
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

DNA Methylation Antibody Panel Pack I

C20000
  • EUR 458.46
  • EUR 270.60
  • 2 x 25 ul

DNMT3A Protein

E11000
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

TET1 Protein (Active)

E12002
  • EUR 387.12
  • EUR 225.50
  • 10 µg
  • 10 ul

DNMT3B Protein 

E14000
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

DNMT1 Protein 

E15000
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

HDAC1 Protein 

E24002
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC10 Protein 

E24003
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC11 Protein 

E24004
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC2 Protein 

E24005
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC3 Protein 

E24006
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC4 Protein 

E24007
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC5 Protein 

E24008
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC6 Protein 

E24009
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC7 Protein 

E24010
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC8 Protein 

E24011
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC9 Protein 

E24012
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

H3F3A Protein 

E35003
  • EUR 174.84
  • EUR 78.10
  • 50 µg
  • 50 ul

H4 Protein 

E35005
  • EUR 174.84
  • EUR 78.10
  • 50 µg
  • 50 ul

SOD1 Protein 

E70000
  • EUR 599.40
  • EUR 365.20
  • 25 µg
  • 25 ul

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Goat Anti-Rat Secondary Antibody, Biotin Conjugated

A12002
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

HRP-Goat Anti-Mouse Secondary Antibody

A12003
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

HRP-Goat Anti-Rabbit Secondary Antibody

A12004
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

EpiMag HT (96-Well) Magnetic Separator

Q10002
  • EUR 416.70
  • EUR 258.50
  • 1/Pack
  • 1/Pack

EpiMag 96-Well Microplate (5/pack) 

Q10003
  • EUR 136.56
  • EUR 52.80
  • 5/pack
  • 5/pack

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

ACE2, His-Tag Protein

E80019
  • EUR 612.70
  • EUR 1437.70
  • 20 ul
  • 100 ul

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag

E80020
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag

E80021
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag

E80022
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag

E80023
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag

E80024
  • EUR 635.80
  • EUR 4995.10
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion

E80026
  • EUR 588.50
  • EUR 823.90
  • 20 ul
  • 50 ul

SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag

E80027
  • EUR 635.80
  • EUR 4087.60
  • 1 ml
  • 100 ul

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028
  • EUR 388.30
  • EUR 860.20
  • 20 ul
  • 100 ul

Stacking platform large 12x12 w flat mat (3.0 separation)

MIX7114 EACH
EUR 150.48

Stacking platform large 12x12 w dimpled mat (3.0 separation)

MIX7116 EACH
EUR 155.04

Rac1 Activation Assay

STA-401-1 20 assays
EUR 908.4
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rap1 Activation Assay Kit

STA-406-1 20 assays
EUR 908.4
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay

STA-407-1 20 assays
EUR 908.4
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Human LOX-1 ELISA Kit

STA-387 96 assays
EUR 936
Description: Cell Biolabs? Human LOX-1 ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human LOX-1 in plasma, serum or cell culture supernatant samples.  The kit has a detection sensitivity limit of 40 pg/mL human LOX-1.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. 

VCAM1 Human, Vascular cell adhesion molecule 1 Human Recombinant Protein, HEK

PROTP19320-1 Regular: 50ug
EUR 380.4
Description: VCAM1 Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 682 amino acids (25-698). VCAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

ESM1 Human, Endothelial Cell-Specific Molecule 1 Human Recombinant Protein, HEK

PROTQ9NQ30-1 Regular: 10ug
EUR 380.4
Description: ESM1 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 20-184) containing 175 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 46.0kDa (calculated). 

STEP antibody

10R-8556 100 ul
EUR 471.6
Description: Mouse monoclonal STEP antibody

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 111.94

Addi platform separators (4 ea) Add 1.25 to platform separation

MIX7118 EACH
EUR 60.42

Methylamp 96 DNA Modification Kit 

P-1008
  • EUR 416.70
  • EUR 253.00
  • 96 Samples
  • 96 Samples

EpiQuik Nuclear Extraction Kit 

OP-0002
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

Nucleic Acid Isolation Enhancer (Glycogen Solution) 

R-1001
  • EUR 164.40
  • EUR 75.90
  • 500 µl
  • 500 ul

Methylamp PCR Enhancer 

R-1002
  • EUR 185.28
  • EUR 90.20
  • 400 Reactions
  • 400 Reactions

Streptavidin: HRP Conjugate

R-1098
  • EUR 218.34
  • EUR 107.80
  • 0.5 ml
  • 0.5 ml

Streptavidin 

R-1100
  • EUR 237.48
  • EUR 121.00
  • 1 mg
  • 1 ml

Protease Inhibitor Cocktail 

R-1101
  • EUR 176.58
  • EUR 79.20
  • 1 ml
  • 1 ml

Streptavidin-Coated Strip Microwell Plate (Flat) 

R-1102
  • EUR 361.02
  • EUR 204.60
  • 5/pk
  • 5/pk

DNA High Binding Solution

R-1103
  • EUR 267.06
  • EUR 140.80
  • 30 ml
  • 30 ml

DTT Solution 

R-1104
  • EUR 131.34
  • EUR 48.40
  • 1 ml, 500 mM
  • 1 ml, 500 mM

PDCD1LG2 Human, Programmed Cell Death 1 Ligand 2 Human Recombinant Protein, Sf9

PROTQ9BQ51-1 Regular: 10ug
EUR 380.4
Description: PDCD1LG2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 423 amino acids (20-200a.a.) and having a molecular mass of 47.7kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).;PDCD1LG2 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Human Alpha 1 Antitrypsin ELISA Kit

PRB-5034 96 assays
EUR 686.4
Description: Our Human Alpha 1 Antitrypsin ELISA Kit is a sandwich ELISA for the detection of human alpha 1 antitrypsin in plasma, serum, lysates, and other biological fluids. Samples or standards are added to an anti-A1AT antibody coated plate followed by the addition of a biotinylated anti-human A1AT antibody. A streptavidin enzyme conjugate is then added for detection. The absorbance is read at 450nm and the level of A1AT in samples is determined based on the provided human A1AT standard curve.

CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit 

OP-0005
  • EUR 474.12
  • EUR 138.60
  • EUR 189.20
  • EUR 292.60
  • 20x96 assays
  • 5x96 assays
  • 10x96 assays
  • 20x96 assays

Rac1 Activation Assay Kit, Trial Size

STA-401-1-T 5 assays
EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size

STA-406-1-T 5 assays
EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size

STA-407-1-T 5 assays
EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Human Alpha 1 Acid Glycoprotein ELISA Kit

PRB-5044 96 assays
EUR 686.4
Description: Our Human ?1AG ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human ?1AG in plasma, serum, urine, cell or tissue lysate samples. The kit has a detection sensitivity limit of 30 pg/mL human ?1AG. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

AmpliStain anti Mouse 1 Step (HRP)

75R-1061 6 ml
EUR 363.6
Description: Mouse antigen staining reagent for use in IHC

AmpliStain anti Rabbit 1 Step (HRP)

75R-1062 6 ml
EUR 363.6
Description: Rabbit antigen staining reagent for use in IHC

AmpliStain anti Goat 1 Step (HRP)

75R-1064 6 ml
EUR 429.6
Description: Goat antigen staining reagent for use in IHC

H. Pylori Rapid Stain (1-Step)

HPS030 30 ml
EUR 75.6

H. Pylori Rapid Stain (1-Step)

HPS500 500 ml
EUR 121.2

H. Pylori Rapid Stain (1-Step)

HPS999 1000 ml
EUR 156

amfiRivert 1-Step RT-PCR Kit

R5003-050 50 rxns
EUR 262.8

amfiRivert 1-Step RT-PCR Kit

R5003-100 100 rxns
EUR 400.8

amfiRivert 1-Step RT-PCR Kit

R5003-200 200 rxns
EUR 562.8

1-Step Polymorphs, Human Cell Separation

 

 

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