1-Step Polymorphs, Human Cell Separation

1-Step Polymorphs, Human Cell Separation 

To Order Contact us: lieven@wlsolutions.be

 

Polymorphprep density Medium
Polymorphprep density Medium

1.Preparation of the human blood samples.
For the best results, use whole blood treated with an anticoagulant
such as EDTA, heparin or citrate. We recommend EDTA (K salt) for
the best result. The blood should be used within two hours of
drawing from the donor. The blood samples and the
Polymorphprep™ solution should be at a temperature of 18-22 °C
and during centrifugation also kept within these limits.

2. Preparation of gradient
Layer 5.0 ml of anti-coagulated whole blood over 5.0 ml of
Polymorphprep™ in a 15 ml centrifuge tube or 18 ml of blood over
18 ml Polymorphprep™ in a 50 ml tube. Take care to avoid mixing
of the blood with the separation fluid. Always use equal volumes of
blood and PolymorphprepTM and select a tube that maintains a
similar geometry of layers to the recommended 5 ml + 5 ml in a 15
ml tube.

3. Separation procedure
Centrifuge the tubes at 500-550 gav for 30 minutes in a swing-out
rotor at 18-22 °C. Allow the rotor to decelerate without the break.
Centrifugation for longer times or higher centrifugal force will result
in the PMNs migrating further down towards the pelleted
erythrocytes.

4. Unloading the gradient
After centrifugation, two leukocyte bands should be visible. The top
band at the sample/medium interface will consist of mononuclear
cells and the lower band of PMNs; the erythrocytes are pelleted.
The cell bands are best harvested using a 2 ml syringe attached to
a flat-tipped metal filling cannula (0.8 mm inner diameter). The
PMNs should be diluted by addition of one volume of 0.45% NaCl
solution or culture medium at 0.5 normal concentration in order to
restore normal osmolality. For further dilutions use normal saline or
culture medium.

5. Washing
The suspension is transferred to a 15 ml tube and the cells pelleted
at approx. 400 g for 10 min (18-22 °C). They are resuspended in
saline or culture medium, sedimented again and then resuspended
in a medium compatible with the subsequent analysis.

Polymorphprep PMN
Polymorphprep PMN

Density Gradient information

Axis Shield Polymorphprep PMN
Axis Shield Polymorphprep PMN

Magnetic Separation Rack

CM103 24 well (1.5 ml/well)
EUR 112

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 661.2
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

K267-50 each
EUR 483.6

Cytosol/Particulate Separation Kit

K2112-50 50 assays
EUR 501.6

Techne Separation Membranes 200x200mm

OVE3038 PK5
EUR 181.2

WHATMAN(R) TG100 SEPARATION GAUZE DIAM

WHA10423029 EACH
EUR 174

OrgFrontier? Viable/Non-Viable Cells Separation Kit

K850-10 each
EUR 652.8

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Corning 500mL LSM (Lymphocyte Separation Medium)

25-072-CV EACH
EUR 156

Addi platform separators (4 ea) Add 1.25 to platform separation

MIX7118 EACH
EUR 74.4

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 52.8

Stacking platform large 12x12 w flat mat (3.0 separation)

MIX7114 EACH
EUR 182.4

Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation)

B3D-STACK 1 PC
EUR 135.8

Stacking platform, large 12"x12" with flat mat (3.0" separation)

BR1000-STACK 1 PC
EUR 135.8

Stacking platform large 12x12 w dimpled mat (3.0 separation)

MIX7116 EACH
EUR 188.4

Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation)

B3D-STACK-D 1 PC
EUR 141

Stacking platform, large 12"x12" with dimpled mat (3.0" separation)

BR1000-STACK-D 1 PC
EUR 141

Stacking Platform, extra large 14"x12" with flat mat (3.0" separation)

BR2000-STACK 1 PC
EUR 147.7

Stacking platform small 10.5x7.5 w flat mat (3.0separation)

MIX7112 EACH
EUR 182.4

Human Whole Blood Polymorphonuclear Cell Isolation Kit

K483-30 each
EUR 451.2

Human Polymorphonuclear Elastase ELISA kit

E01P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA kit

E01P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA kit

E01P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA Kit

ELA-E1310h 96 Tests
EUR 988.8

Separating Funnel PP 500ml

FUN21060 PK2
EUR 93.6

50ml Separating Funnel

FUN4123 EACH
EUR 104.4

Pyrex 1L Separating Funnel

FUN4126 EACH
EUR 139.2

Pyrex 2L Separating Funnel

FUN4128 EACH
EUR 187.2

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

201-12-0852 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

CN-04588H1 96T
EUR 573.6

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

CN-04588H2 48T
EUR 394.8

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

GA-E0868HM-48T 48T
EUR 346.8

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

GA-E0868HM-96T 96T
EUR 559.2

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

QY-E03725 96T
EUR 480

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

YLA1660HU-48T 48T
EUR 435

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

YLA1660HU-96T 96T
EUR 562.5

Separating Funnel Pear 1000ml

FUN20650 PK2
EUR 205.2

Separating Funnel Pear 50ml

FUN2081 EACH
EUR 74.4

Separating Funnel Pear 100ml

FUN2083 EACH
EUR 85.2

Separating Funnel Pear 250ml

FUN2085 EACH
EUR 90

2L P/Shaped Separating Funnel

FUN4310 EACH
EUR 207.6

Separating Funnel Holder

STA1094 EACH
EUR 39.24

Separating funnel 100ML PTFE Key

8S149/100 EACH
EUR 45.6

Separating Funnel 500ml PTFE Key

8S149500 EACH
EUR 56.4

Pyrex 100ml Separating Funnel

FUN4120 EACH
EUR 104.4

Pyrex 250ml Separating Funnel

FUN4122 EACH
EUR 114

Pyrex 500ml Separating Funnel

FUN4124 EACH
EUR 120

Funnel Pyrex Separating Pear 5L

FUN4312 EACH
EUR 309.6

Long Form Separating Funnel 50ml

8S14950 EACH
EUR 44.4

50ml P/Shaped Separating Funnel

FUN4300 EACH
EUR 110.4

Nalgene 125ml Separating Funnel PP

FUN4180 EACH
EUR 97.2

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 182.4

MHC Class II Polymorphic Antibody

abx415487-025ml 0.25 ml
EUR 727.2

MHC Class II DQ DR Polymorphic Antibody (RPE)

abx413858-100tests 100 tests
EUR 861.6

MHC Class II DQ DR Polymorphic Antibody (FITC)

abx413861-01mg 0.1 mg
EUR 777.6

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Funnel Separating Conical Ptfe Stopcock 100ml

E4606 EACH
EUR 33.6

Funnel Separating Conical Ptfe Stopcock 250ml

E4607 EACH
EUR 43.2

Simax Separating Funnel Cylindrical Open 50ml

FUN2060 EACH
EUR 46.8

Simax Separating Funnel Cylindrical Open 100ml

FUN20660 PK4
EUR 180

Pyrex Separating Funnel 100ml Glass Stopcock Pear

FUN4322 EACH
EUR 117.6

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Polymorphonuclear Elastase ELISA kit

E08P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Polymorphonuclear Elastase ELISA kit

E08P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Polymorphonuclear Elastase ELISA kit

E08P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Polymorphonuclear Elastase ELISA kit

E07P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Polymorphonuclear Elastase ELISA kit

E07P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Polymorphonuclear Elastase ELISA kit

E07P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Polymorphonuclear Elastase ELISA kit

E06P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Polymorphonuclear Elastase ELISA kit

E06P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Polymorphonuclear Elastase ELISA kit

E06P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pyrex Morbank Separating Funnel 1L Glass Stopcock Pear

FUN4328 EACH
EUR 148.8

Mouse Polymorphonuclear Elastase ELISA kit

E03P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Polymorphonuclear Elastase ELISA kit

E03P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Polymorphonuclear Elastase ELISA kit

E03P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Polymorph Nuclear Leukocytes ELISA Kit

QY-E30302 96T
EUR 448.8

Rabbit Polymorphonuclear Elastase ELISA kit

E04P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Polymorphonuclear Elastase ELISA kit

E04P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Polymorphonuclear Elastase ELISA kit

E04P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Polymorphonuclear Elastase ELISA kit

E09P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Polymorphonuclear Elastase ELISA kit

E09P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Polymorphonuclear Elastase ELISA kit

E09P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pyrex Pear Shaped Separating Funnel with PTFE Stopcock 250ml

FUN4324 EACH
EUR 122.4

Guinea pig Polymorphonuclear Elastase ELISA kit

E05P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Polymorphonuclear Elastase ELISA kit

E05P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Polymorphonuclear Elastase ELISA kit

E05P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100698 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100699 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100700 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100701 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100702 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100703 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100704 100 µg
EUR 424.8

Anti-MUC1/Polymorphic epithelial mucin antibody

STJ16100705 100 µg
EUR 424.8

Recombinant HPV-18 L1 Protein (Hansenula polymorpha)

VAng-0555Lsx-inquire inquire Ask for price
Description: HPV type 18 L1 Protein, recombinant protein from Hansenula polymorpha. 2.22 mg/mL.

Recombinant HPV-16 L1 Protein (Hansenula polymorpha)

VAng-0556Lsx-inquire inquire Ask for price
Description: HPV type 16 L1 Protein, recombinant protein from Hansenula polymorpha. 2.75 mg/mL.

Brand squibb separating funnel, pp stopper, ungraduated, ptfe key, 250 ml

Z330930-2EA PK2
EUR 165.81

Canine Polymorphonuclear Elastase,PMN Elastase ELISA Kit

QY-E70120 96T
EUR 511.2

Recombinant HPV-16 L1 Protein (Full Length) (Hansenula polymorpha)

VAng-Lsx0266-inquire inquire Ask for price
Description: HPV type 16 L1 Protein (full length), recombinant protein from Hansenula polymorpha.

Chlamydia pneumoniae CPN-Ag Polymorphic membrane protein D, PmpD

E63H001 100ug
EUR 458.4

Sheep Polymorphonuclear Elastase/Neutrophil elastase (ELANE) ELISA Kit

abx364201-96tests 96 tests
EUR 1111.2

Chicken Polymorphonuclear Elastase/Neutrophil elastase (ELANE) ELISA Kit

abx355687-96tests 96 tests
EUR 990

1-Step Polymorphs, Human Cell Separation

 

 

Recente berichten

Categorieën

september 2023
M D W D V Z Z
 123
45678910
11121314151617
18192021222324
252627282930  

Pagina’s

Tags