1-Step Polymorphs, Human Cell Separation

1-Step Polymorphs, Human Cell Separation 

To Order Contact us: lieven@wlsolutions.be

 

Polymorphprep density Medium
Polymorphprep density Medium

1.Preparation of the human blood samples.
For the best results, use whole blood treated with an anticoagulant
such as EDTA, heparin or citrate. We recommend EDTA (K salt) for
the best result. The blood should be used within two hours of
drawing from the donor. The blood samples and the
Polymorphprep™ solution should be at a temperature of 18-22 °C
and during centrifugation also kept within these limits.

2. Preparation of gradient
Layer 5.0 ml of anti-coagulated whole blood over 5.0 ml of
Polymorphprep™ in a 15 ml centrifuge tube or 18 ml of blood over
18 ml Polymorphprep™ in a 50 ml tube. Take care to avoid mixing
of the blood with the separation fluid. Always use equal volumes of
blood and PolymorphprepTM and select a tube that maintains a
similar geometry of layers to the recommended 5 ml + 5 ml in a 15
ml tube.

3. Separation procedure
Centrifuge the tubes at 500-550 gav for 30 minutes in a swing-out
rotor at 18-22 °C. Allow the rotor to decelerate without the break.
Centrifugation for longer times or higher centrifugal force will result
in the PMNs migrating further down towards the pelleted
erythrocytes.

4. Unloading the gradient
After centrifugation, two leukocyte bands should be visible. The top
band at the sample/medium interface will consist of mononuclear
cells and the lower band of PMNs; the erythrocytes are pelleted.
The cell bands are best harvested using a 2 ml syringe attached to
a flat-tipped metal filling cannula (0.8 mm inner diameter). The
PMNs should be diluted by addition of one volume of 0.45% NaCl
solution or culture medium at 0.5 normal concentration in order to
restore normal osmolality. For further dilutions use normal saline or
culture medium.

5. Washing
The suspension is transferred to a 15 ml tube and the cells pelleted
at approx. 400 g for 10 min (18-22 °C). They are resuspended in
saline or culture medium, sedimented again and then resuspended
in a medium compatible with the subsequent analysis.

Polymorphprep PMN
Polymorphprep PMN

Density Gradient information

Axis Shield Polymorphprep PMN
Axis Shield Polymorphprep PMN

Lymphocyte separation medium (human)

abx082102-1ml 1 ml Ask for price

Lymphocyte separation medium (human)

abx082102-200l 200 µl Ask for price

Hybridoma specific B cell separation Solution (10x)

E28TYA0601 100ml
EUR 190.48

Magnetic Separation Rack

CM101 32 well (200 μl/well)
EUR 140

Magnetic Separation Rack

CM103 24 well (1.5 ml/well)
EUR 140

Lymphocyte Separation Medium, Sterile

40120344-1 100 ml
EUR 25.62

Lymphocyte Separation Medium, Sterile

40120344-2 250 ml
EUR 54.64

Lymphocyte Separation Medium, Sterile

40120344-3 500 ml
EUR 90.31

Lymphocyte Separation Solution

20828-15 500ML
EUR 207.9

Lymphocyte Separation Solution

20828-44 100ML
EUR 46.2

Lymphocyte Separation Solution

20839-04 100ML
EUR 57.4

Bromoform for Mineral Separation

GRM10947-250ML 1 unit
EUR 66.62
Description: Bromoform for Mineral Separation

genesig magnetic separation rack

Z-genesigEASY-MR n/a
EUR 539

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 433
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

GWB-AXR181 50 assays Ask for price

Cytosol/Particulate Separation Kit

K2112-50 50 assays
EUR 605
Description: Rapidly separates cytosol from particulate fractions

Cytosol/Particulate Separation Kit

K267-50 each
EUR 483.6

OrgFrontier? Viable/Non-Viable Cells Separation Kit

K850-10 each
EUR 652.8

Techne Separation Membranes 200x200mm - PK5

OVE3038 PK5
EUR 203.85

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Lymphosep. Lymphocyte Separation Media - 100ml

LM-T1702/100 100ml
EUR 10.78

Lymphosep. Lymphocyte Separation Media - 500ml

LM-T1702/500 500ml
EUR 44.88

WHATMAN(R) TG100 SEPARATION GAUZE DIAM - EACH

WHA10423029 EACH
EUR 195.75

OOTA00785-100ML - Lymphocyte Separation Medium

OOTA00785-100ML 100mL
EUR 59

Corning 500mL LSM (Lymphocyte Separation Medium) - EACH

25-072-CV EACH
EUR 175.5

Addi platform separators (4 ea) Add 1.25 to platform separation - EACH

MIX7118 EACH
EUR 83.7

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 54.38

Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation)

B3D-STACK 1 PC
EUR 139.87

Stacking platform, large 12"x12" with flat mat (3.0" separation)

BR1000-STACK 1 PC
EUR 139.87

50mL Peripheral Blood Lymphocyte Separation Tube, 25/pk, 100/cs

602851 100 pcs/cs
EUR 194.37
Description: 50mL Peripheral Blood Lymphocyte Separation Tube, 25/pk, 100/cs

50mL Peripheral Blood Lymphocyte Separation Tube,25/pk, 500/cs

602852 500 pcs/cs
EUR 710.16
Description: 50mL Peripheral Blood Lymphocyte Separation Tube,25/pk, 500/cs

15mL Peripheral Blood Lymphocyte Separation Tube, 50/pk, 150/cs

601851 150 pcs/cs
EUR 212.19
Description: 15mL Peripheral Blood Lymphocyte Separation Tube, 50/pk, 150/cs

15mL Peripheral Blood Lymphocyte Separation Tube, 50/pk, 500/cs

601852 500 pcs/cs
EUR 560.23
Description: 15mL Peripheral Blood Lymphocyte Separation Tube, 50/pk, 500/cs

Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation)

B3D-STACK-D 1 PC
EUR 145.23

Stacking platform large 12x12 w flat mat (3.0 separation) - EACH

MIX7114 EACH
EUR 205.2

Stacking platform, large 12"x12" with dimpled mat (3.0" separation)

BR1000-STACK-D 1 PC
EUR 145.23

Stacking platform large 12x12 w dimpled mat (3.0 separation) - EACH

MIX7116 EACH
EUR 211.95

Stacking Platform, extra large 14"x12" with flat mat (3.0" separation)

BR2000-STACK 1 PC
EUR 152.13

TFF-MV- tangential flow filter for large EV separation and recovery

HBM-TFF-MV 1 concentrator
EUR 181.44

Stacking platform small 10.5x7.5 w flat mat (3.0separation) - EACH

MIX7112 EACH
EUR 205.2

Human Whole Blood Polymorphonuclear Cell Isolation Kit

K483-30 each
EUR 451.2

Human Polymorphonuclear Elastase ELISA kit

E01P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA kit

E01P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA kit

E01P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Polymorphonuclear Elastase ELISA kit

E01A7093 96T
EUR 700
Description: ELISA

Human Polymorphonuclear Elastase ELISA Kit

ELA-E1310h 96 Tests
EUR 988.8

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

201-12-0852 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

GA-E0868HM-48T 48T
EUR 346.8

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

GA-E0868HM-96T 96T
EUR 559.2

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

CN-04588H1 96T
EUR 573.6

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

CN-04588H2 48T
EUR 394.8

Human Polymorphonuclear Elastase(PMN Elastase)ELISA Kit

QY-E03725 96T
EUR 480

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

SL1436Hu 96 Tests
EUR 468

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

YLA1660HU-48T 48T Ask for price

Human Polymorphonuclear Elastase,PMN Elastase ELISA Kit

YLA1660HU-96T 96T Ask for price

Human Polymorphonuclear Elastase, PMN Elastase GENLISA ELISA

KBH0851 1 x 96 wells
EUR 286

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100 200 rcs
EUR 249

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100L 2000rcs
EUR 1599

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100XL 10000rcs Ask for price

Separating Gel Buffer 4X

10530008-1 100 mL
EUR 38.93

Separating Gel Buffer 4X

10530008-2 250 mL
EUR 63.78

Separating Gel Buffer 4X

10530008-3 500 mL
EUR 86.02

Separating Funnel PP 500ml - PK2

FUN21060 PK2
EUR 105.3

50ml Separating Funnel - EACH

FUN4123 EACH
EUR 117.45

MHC Class II Polymorphic Antibody

abx415487-025ml 0.25 ml
EUR 727.2

MHC Class II DQ DR Polymorphic Antibody (PE)

abx413858-200l 200 µl
EUR 637.5

MHC Class II Polymorphic Antibody

abx415487-200l 200 µl
EUR 537.5

Separating Funnel Pear 1000ml - PK2

FUN20650 PK2
EUR 230.85

Pyrex 1L Separating Funnel - EACH

FUN4126 EACH
EUR 156.6

Pyrex 2L Separating Funnel - EACH

FUN4128 EACH
EUR 210.6

MHC Class II DQ DR Polymorphic Antibody (RPE)

abx413858-100tests 100 tests
EUR 861.6

Separating Funnel Pear 50ml - EACH

FUN2081 EACH
EUR 83.7

Separating Funnel Pear 100ml - EACH

FUN2083 EACH
EUR 95.85

Separating Funnel Pear 250ml - EACH

FUN2085 EACH
EUR 101.25

2L P/Shaped Separating Funnel - EACH

FUN4310 EACH
EUR 233.55

Separating Funnel Holder - EACH

STA1094 EACH
EUR 44.15

MHC Class II DQ DR Polymorphic Antibody (FITC)

abx413861-01mg 0.1 mg
EUR 777.6

MHC Class II DQ DR Polymorphic Antibody (FITC)

abx413861-200l 200 µl
EUR 575

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

Pyrex 100ml Separating Funnel - EACH

FUN4120 EACH
EUR 117.45

Pyrex 250ml Separating Funnel - EACH

FUN4122 EACH
EUR 128.25

Pyrex 500ml Separating Funnel - EACH

FUN4124 EACH
EUR 135

Funnel Pyrex Separating Pear 5L - EACH

FUN4312 EACH
EUR 348.3

Separating funnel 100ML PTFE Key - EACH

8S149/100 EACH
EUR 51.3

Separating Funnel 500ml PTFE Key - EACH

8S149500 EACH
EUR 63.45

Sheep MHC Class II Antibody (DR Polymorphic)

GWB-Q01342 0.25 mg Ask for price

Sheep MHC Class II Antibody (DR Polymorphic)

GWB-Q01343 0.25 mg Ask for price

50ml P/Shaped Separating Funnel - EACH

FUN4300 EACH
EUR 124.2

Long Form Separating Funnel 50ml - EACH

8S14950 EACH
EUR 49.95

Sheep MHC Class II Antibody (DQ DR Polymorphic) (RPE)

GWB-Q01346 100 TESTS Ask for price

Nalgene 125ml Separating Funnel PP - EACH

FUN4180 EACH
EUR 109.35

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 182.4

Sheep MHC Class II Antibody (DR beta Polymorphic)

GWB-4F3017 2 ml Ask for price

Guinea Pig MHC Class II Antibody (polymorphic)

GWB-Q00952 0.25 ml Ask for price

Sheep MHC Class II Antibody (DQ DR Polymorphic) (FITC)

GWB-Q01344 0.1 mg Ask for price

Sheep MHC Class II Antibody (DQ DR Polymorphic) (FITC)

GWB-Q01345 0.1 mg Ask for price

HiPer® Random Amplification of Polymorph

HTBM031-5PR 1 unit
EUR 82.29
Description: HiPer® Random Amplification of Polymorph

HiPer® Single Nucleotide Polymorphism (S

HTBM034-10PR 1 unit
EUR 65.04
Description: HiPer® Single Nucleotide Polymorphism (S

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Polymorphonuclear Elastase ELISA kit

E02P0690-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Polymorphonuclear Elastase ELISA kit

E08P0690-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Polymorphonuclear Elastase ELISA kit

E08P0690-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Polymorphonuclear Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

1-Step Polymorphs, Human Cell Separation

 

 

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