Design & Development of Laboratory Test Kits for IVD
Comparability of Industrial ELISA Kits
Comparability of Industrial ELISAKits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based mostly Immunoassay for Detecting a Urine-Based mostly Bladder-Most cancers-Related Diagnostic Signature.
The power to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of scientific assessments composed of a number of biomarkers. We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers). Banked urine samples collected from Kyoto and Nara Universities had been in comparison with histologically decided bladder most cancers.
The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial progress factor-VEGF) had been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) based on the producer’s technical specs.
The vary for detecting every biomarker was improved within the multiplex assays, though the decrease restrict of quantification (LLOQ) was sometimes decrease within the industrial ELISA kits. The realm beneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA). The sensitivities and specificities for MBA had been 0.93 and 0.95, respectively, and for MEA had been 0.85 and 0.80, respectively.
Accuracy, optimistic predictive values (PPV), and damaging predictive values (NPV) for MBA had been 0.94, 0.95, and 0.93, respectively, and for MEA had been 0.83, 0.81, and 0.84, respectively. Based mostly on these encouraging preliminary information, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environment friendly and extremely correct instrument to quantitate a number of proteins inside biologic specimens.
Description: Quantitativesandwich ELISA kit for measuring Human heme oxygenase 1, HO-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat heme oxygenase 1, HO-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse heme oxygenase 1, HO-1 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human HO-1/HMOX1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human heme oxygenase 1, HO-1 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat heme oxygenase 1, HO-1 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse heme oxygenase 1, HO-1 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human heme oxygenase 2, HO-2 in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Heme Oxygenase 1 (HO-1) is an enzyme in endoplasmic reticulum that belongs to the heme oxygenase family. HO-1 cleaves the heme ring at the alpha methene bridge to form Biliverdin. Biliverdin is subsequently converted to Bilirubin by Biliverdin reductase. In physiological state, the highest activity of HO-1 is found in the spleen, where senescent erythrocytes are sequestrated and destroyed. HO-1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. HO-1 is involved in the regulation of cardiovascular function and response to a variety of stressors. Defects in HO-1 are the cause of Heme Oxygenase 1 deficiency, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues.
Description: His tagged recombinant rat HO-1 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.
Description: His tagged recombinant rat HO-1 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.
Description: His tagged recombinant rat HO-1 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
Description: HO-3867 is a novel curcumin analog and a selective inhibitor of STAT3. [1]STAT3 (signal transducer and activator of transcription 3) belongs to the STAT protein family. It mediates various gene expressions for numerous cellular functions, including cell growth, division and apoptosis.
Description: HO-3867 is a novel curcumin analog and a selective inhibitor of STAT3. [1]STAT3 (signal transducer and activator of transcription 3) belongs to the STAT protein family. It mediates various gene expressions for numerous cellular functions, including cell growth, division and apoptosis.
Description: HO-3867 is a novel curcumin analog and a selective inhibitor of STAT3. [1]STAT3 (signal transducer and activator of transcription 3) belongs to the STAT protein family. It mediates various gene expressions for numerous cellular functions, including cell growth, division and apoptosis.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HMOX1 / HO-1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HMOX1 / HO-1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HMOX1 / HO-1 . This antibody is tested and proven to work in the following applications:
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is not conjugated.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is not conjugated.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 390.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 488.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 565.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 594.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 633.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 655.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 680.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with ATTO 700.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with Alkaline Phosphatase.
Description: A monoclonal antibody from clone 1F12-A6 against Human | Mouse | Rat | Bovine | Chicken HO-1. The host species for the production of this antibody is Mouse. The antigen used for immunization is Human HO-1 synthetic peptide, amino acids 1-30. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:100). This MAb for HO-1 is conjugated with APC.
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Comparability of Speedy Anti-HCV Multi-sure Package with Gold Customary ELISA.
To match the diagnostic yield of Multi-sure fast HCV (hepatitis C virus) package with ELISA.Comparative research.Pakistan Well being Analysis Council, specialised analysis heart for gastroenterology and hepatology, from August 2016 to January 2017.A modified fast anti-HCV package was in contrast with ELISA.
This fast package is multi-parameter qualitative immune chromatographic package for the in-vitro detection of antibodies to HCV in human blood. Sufferers who got here to PHRC, had been examined utilizing anti-HCV ELISA, and their check was run concurrently on multi-sure HCV fast package had been included within the research. Every optimistic and damaging pattern was included on this research. SPSS software program was tailored for information evaluation.
A complete of 420 samples had been collected. Amongst them, 255 (61%) had been of male and 165 (39%) had been of feminine sufferers. Imply age was 35 ± 14.33 years. All of the samples run for anti-HCV on ELISA had been additionally run on multi-sure fast package. It’s evident that 22.4% had been reactive on ELISA and 23.6% had been reactive on fast package, whereas 75.5% had been non-reactive on ELISA and 68.1% had been non-reactive on fast package. Borderline optimistic outcomes had been seen in 2.1% on ELISA and 5.0% on fast package. Sensitivity of fast package was 87.2%, specificity 89.3% with 82.8% optimistic predictive worth and 98.9% damaging predictive worth.Multi-sure package confirmed considerably, much less non-reactive and extra borderline outcomes as in comparison with ELISA.
Comparability of multi-sure fast package with ELISA confirmed that core antibody can be utilized as an alternate marker for ELISA. Different non-structural proteins together with NS3, NS4 and NS5 had been discovered to be much less important. So, it’s concluded that this fast package will not be really helpful in its place of ELISA, apart from locations the place ELISA will not be out there.
Evaluation of luteinizing hormone (LH): Validation of a industrial ELISApackage for LH evaluation and quantification in doping management samples.
Luteinizing hormone (LH) is a dimeric glycoprotein produced and secreted by the pituitary gland, with a molecular weight of roughly 30 000 Da. The primary scientific use for exogenous LH-administration is often linked to the therapy of infertility, in each women and men. The specified impact of LH misuse in sport is because of the enhancement of testicular manufacturing of testosterone.
Elevated LH ranges can also point out the utilization of gonadotropin-releasing components or estrogen blockers. Subsequently, LH is listed by the World Anti-Doping Company (WADA) as a prohibited substance in male athletes, and based on the WADA technical doc, laboratories ought to decide the the full LH concentrations in urine. The TD lists two totally different assays which can be appropriate for measuring complete LH in urine, Delfia and Siemens Immulite.
Different fit-for-purpose assays can be utilized, so long as they’re able to detecting complete LH in urine. In case an assay not listed within the TD might be used, population-based reference values must be decided within the validation process. On this research a brand new immunoassay was validated for the measurement of LH in urine. The assay (AccuBind ELISA Microwells, Luteinizing Hormone, Monobind Inc.), initially supposed for serum, confirmed satisfactory sensitivity and was confirmed fit-for-purpose in routine doping management. Inhabitants-based distribution of the assay was in good settlement with the outcomes of Delfia and Immulite assays, for which the method-specific cut-off-values are 40 IU/L (Delfia) and 60 IU/L (Siemens Immulite).
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma or other biological fluids.
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Description: Among numerous types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the byproducts of oxidative DNA damage, is physiologically formed and enhanced by chemical carcinogens. Our OxiSelect Oxidative DNA Damage ELISA Kit (8-hydroxydeoxyguanosine assay) provides a powerful method for rapid, sensitive quantitation of 8-OHdG in DNA samples.
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (5 plates)
Description: Among numerous types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the byproducts of oxidative DNA damage, is physiologically formed and enhanced by chemical carcinogens. Our OxiSelect Oxidative DNA Damage ELISA Kit (8-hydroxydeoxyguanosine assay) provides a powerful method for rapid, sensitive quantitation of 8-OHdG in DNA samples.
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size
Description: Among numerous types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the byproducts of oxidative DNA damage, is physiologically formed and enhanced by chemical carcinogens. Our OxiSelect Oxidative DNA Damage ELISA Kit (8-hydroxydeoxyguanosine assay) provides a powerful method for rapid, sensitive quantitation of 8-OHdG in DNA samples.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 8-Hydroxydeoxyguanosine (8-OHdG) in serum, plasma and other biological fluids.
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 8-Hydroxydeoxyguanosine (8-OHdG) in serum, plasma and other biological fluids.
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 8-Hydroxydeoxyguanosine (8-OHdG) in serum, plasma and other biological fluids.
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General 8-Hydroxydeoxyguanosine (8-OHdG) in serum, plasma and other biological fluids.
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitativecompetitive ELISA kit for measuring Human 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Human 8-Hydroxydeoxyguanosine (8-OHdG) in samples from serum, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat 8-Hydroxy-desoxyguanosine, 8-OHdG in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat 8-Hydroxy-desoxyguanosine, 8-OHdG in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Mouse 8-Hydroxy-desoxyguanosine, 8-OHdG in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Mouse 8-Hydroxy-desoxyguanosine, 8-OHdG in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive Inhibition ELISA kit for detection of 8-Hydroxydeoxyguanosine from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples.
Description: The OxiSelect Nitrosative DNA/RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-nitroguanine in urine, serum, or plasma samples.
Description: Quantitative sandwich ELISA for measuring Human 8-Hydroxy-desoxyguanosine (8-OHdG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human 8-Hydroxy-desoxyguanosine (8-OHdG)
Description: Quantitative sandwich ELISA for measuring Human 8-Hydroxy-desoxyguanosine (8-OHdG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human 8-Hydroxy-desoxyguanosine (8-OHdG)
Description: Quantitative sandwich ELISA for measuring Human 8-Hydroxy-desoxyguanosine (8-OHdG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
