SUMO (small ubiquitin-like MOs) Protease 1 (Ulp1, Saccharomyces cerevisiae Ubl-specific protease 1) is a highly active cysteine protease. It is very specific as it recognizes the tertiary structure of the ubiquitin-like protein (UBL), SUMO (Smt3), rather than its amino acid sequence.
The SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance the production of functional proteins in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility.
Recombinant Protease 1 EZCut ™ SUMO from BioVision containing an N-terminal His tag is expressed in E. coli and purified by proprietary chromatographic techniques. EZCut SUMO Protease 1 can be used to cleave the SUMO protein tag from recombinant SUMO fusion proteins.
The optimum temperature for cleavage is 30 ° C; however, the enzyme is active over wide ranges of temperature and pH. After completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using Ni chelating resin (Cat. # 6562-1, 10, 100, 500).