Custom CRISPR All-in-one non-viral

Custom CRISPR All-in-one non-viral 

To Order Contact us: lieven@wlsolutions.be

Custom siRNA
M1253-6	each	EUR 548.4

Custom siRNA
M1253-7	each	EUR 568.8

Custom siRNA
M1253-8	each	EUR 594

Custom siRNA
M1253-9	each	EUR 633.6

Custom Color
M-CEMG2-CustomColor	1 SERVICE	Ask for price
Description: Custom Color; Pucks - Available colors: Blue, Black, Brown, Gold , Green, Grey, Orange, Purple, Red, Violet, Rainbow - One of each

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests	25 tests	EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

One Tube Viral DNA Isolation Buffer
VT4785	50Preps	EUR 224.44

One Tube Viral DNA Isolation Buffer
VT4786	100preps	EUR 388.86

Custom Neg Control siRNA
M1256-1	each	EUR 333.6

Custom Pos Control siRNA
M1258-1	each	EUR 333.6

Equine viral arteritis One-Step PCR kit
Oneq-V160-100R	100T	EUR 1932

Equine viral arteritis One-Step PCR kit
Oneq-V160-150R	150T	EUR 2646

Equine viral arteritis One-Step PCR kit
Oneq-V160-50R	50T	EUR 1410

Feline viral rhinotracheitis One-Step PCR kit
Oneq-V170-100D	100T	EUR 1039.2

Feline viral rhinotracheitis One-Step PCR kit
Oneq-V170-150D	150T	EUR 1177.2

Feline viral rhinotracheitis One-Step PCR kit
Oneq-V170-50D	50T	EUR 861.6

Custom Peptide Synthesis order form
Pepord-1	1	Ask for price

Custom Engraving of Uni-Puck
M-CP-CENGR	1 SERVICE	Ask for price
Description: Custom Engraving of Uni-Puck

Bovine viral diarrhea virus One-Step PCR kit
Oneq-V223-100R	100T	EUR 1932

Bovine viral diarrhea virus One-Step PCR kit
Oneq-V223-150R	150T	EUR 2646

Bovine viral diarrhea virus One-Step PCR kit
Oneq-V223-50R	50T	EUR 1410

Viral hemorrhagic septicemia virus One-Step PCR kit
Oneq-V264-100R	100T	EUR 1932

Viral hemorrhagic septicemia virus One-Step PCR kit
Oneq-V264-150R	150T	EUR 2646

Viral hemorrhagic septicemia virus One-Step PCR kit
Oneq-V264-50R	50T	EUR 1410

Rabbit Viral Hemorrhagic Disease One-Step PCR kit
Oneq-V387-100R	100T	EUR 1932

Rabbit Viral Hemorrhagic Disease One-Step PCR kit
Oneq-V387-150R	150T	EUR 2646

Rabbit Viral Hemorrhagic Disease One-Step PCR kit
Oneq-V387-50R	50T	EUR 1410

Non-Viral PhiC31 Integrase Mouse iPSC Reprogramming System (includes FC200PA-1 and FC305A-1)
FC300A-1	1 Kit	EUR 2082

Scrambled sgRNA CRISPR/Cas9 All-in-One Lentivector
K010	1.0 ug	EUR 184.8

Custom Antibodies to Proteins Order form
CAB-F1	1	Ask for price

Custom drilling for BSW01 (sold separately)
BSW01-DR	1 PC	EUR 182.32

Published references using ADI custom services
CPUB-1	1	Ask for price

Rat Furin (Furin) ELISA Kit (CUSTOM)
ELI-32535r	96 Tests	EUR 1063.2

Human GPHN (Gephyrin) ELISA Kit (CUSTOM)
ELI-32627h	96 Tests	EUR 988.8

Bovine Furin (Furin) ELISA Kit (CUSTOM)
ELI-30801b	96 Tests	EUR 1113.6

Feline Viral Rhinotracheitis protein
30-1259	1 mg	EUR 548.4
Description: Purified native Feline Viral Rhinotracheitis protein

Viral DNA / RNA Kit
20-abx098090	EUR 126.00 EUR 343.20	2 rxns 50 rxns

Viral DNA / RNA Kit
abx098872-50rxns	50 rxns	EUR 444

MIP-2, CXCL2, viral
RC392-13	50ug	EUR 203.56

Zenoquick Viral RNA Kit
Z5013-050	50 preps	EUR 416.4

Zenoquick Viral RNA Kit
Z5013-200	4x50 preps	EUR 1146

MIP-2, Viral Recombinant
P1295-10	each	EUR 210

MIP-2, Viral Recombinant
P1295-50	each	EUR 444

Bovine Viral Diarrhea Virus 2 One-Step PCR kit
Oneq-V236-100R	100T	EUR 1932

Bovine Viral Diarrhea Virus 2 One-Step PCR kit
Oneq-V236-150R	150T	EUR 2646

Bovine Viral Diarrhea Virus 2 One-Step PCR kit
Oneq-V236-50R	50T	EUR 1410

TDOR sgRNA CRISPR/Cas9 All-in-One Lentivector set ()
K9000805	3 x 1.0 ug	EUR 451.2

Custom Peptide Synthesis and Antiibodies Order form
CAP-F2	1	Ask for price

Mouse SH3GL2 (Endophilin- A1) ELISA Kit (CUSTOM)
ELI-52963m	96 Tests	EUR 1038

Mouse RTN1 (Reticulon- 1) ELISA Kit (CUSTOM)
ELI-53344m	96 Tests	EUR 1038

Anti-miR-582-5p MZIP Construct CUSTOM
MZIP582-PA-1	Bacterial Streak	EUR 820.8

amfiSure PCR Premix, Custom Order, Including Primer
P0318-960	10X96 rxns	EUR 2587.2

amfiSure PCR Premix, Custom Order, Without Primer
P0319-960	10X96 rxns	EUR 2587.2

Human MB21D1 (Protein MB21D1) ELISA Kit (CUSTOM)
ELI-43014h	96 Tests	EUR 988.8

Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-34287m	96 Tests	EUR 1038

Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-34330h	96 Tests	EUR 988.8

Bovine RCN3 (Reticulocalbin- 3) ELISA Kit (CUSTOM)
ELI-35646b	96 Tests	EUR 1113.6

Human RCN3 (Reticulocalbin- 3) ELISA Kit (CUSTOM)
ELI-41187h	96 Tests	EUR 988.8

Chicken SH3GL2 (Endophilin- A1) ELISA Kit (CUSTOM)
ELI-19848c	96 Tests	EUR 1113.6

Human SERPINB10 (Serpin B10) ELISA Kit (CUSTOM)
ELI-20035h	96 Tests	EUR 988.8

Rat RTN1 (Reticulon- 1) ELISA Kit (CUSTOM)
ELI-20212r	96 Tests	EUR 1063.2

Mouse FAM3D (Protein FAM3D) ELISA Kit (CUSTOM)
ELI-20793m	96 Tests	EUR 120

Human RTN1 (Reticulon- 1) ELISA Kit (CUSTOM)
ELI-29470h	96 Tests	EUR 988.8

Bovine SERPINB10 (Serpin B10) ELISA Kit (CUSTOM)
ELI-30151b	96 Tests	EUR 1113.6

Mouse SERPINB10 (Serpin B10) ELISA Kit (CUSTOM)
ELI-30152m	96 Tests	EUR 1038

Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-48960r	96 Tests	EUR 1063.2

Mouse RCN3 (Reticulocalbin-3) ELISA Kit (CUSTOM)
ELI-14162m	96 Tests	EUR 1038

Mouse ATL2 (Atlastin- 2) ELISA Kit (CUSTOM)
ELI-11367m	96 Tests	EUR 1038

Human ATL2 (Atlastin- 2) ELISA Kit (CUSTOM)
ELI-11415h	96 Tests	EUR 988.8

Human FAM171A1 (Protein FAM171A1) ELISA Kit (CUSTOM)
ELI-09821h	96 Tests	EUR 988.8

Rat SH3GL2 (Endophilin- A1) ELISA Kit (CUSTOM)
ELI-18654r	96 Tests	EUR 1063.2

EnzyFluo™ Custom Cell-Based ELISA Kit
EFC1M-100	100	EUR 359
Description: A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary mouse antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

EnzyFluo™ Custom Cell-Based ELISA Kit
EFC2R-100	100	EUR 359
Description: A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary rabbit antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

ART 10: non-filtered: non-sterile
PIP2248	PK4800	EUR 471.96

ART 10: non-filtered: non-sterile
PIP2260	PK4800	EUR 432.06

ART 200: non-filtered: non-sterile
PIP2274	PK4800	EUR 486.78

ART 200: non-filtered: non-sterile
PIP2276	PK4800	EUR 432.06

ART 300: non-filtered: non-sterile
PIP2278	PK4800	EUR 593.94

ART 300: non-filtered: non-sterile
PIP2280	PK4800	EUR 544.92

ART 1000: non-filtered: non-sterile
PIP2282	PK3072	EUR 411.54

ART 1000: non-filtered: non-sterile
PIP2284	PK3072	EUR 356.82

ART 10: non-filtered: non-sterile
PIP2286	PK4800	EUR 441.18

ART 10: non-filtered: non-sterile
PIP2288	PK4800	EUR 388.74

ART 200: non-filtered: non-sterile
PIP2294	PK4800	EUR 441.18

ART 200: non-filtered: non-sterile
PIP2296	PK4800	EUR 375.06

ART 300: non-filtered: non-sterile
PIP2298	PK4800	EUR 550.62

ART 300: non-filtered: non-sterile
PIP2372	PK4800	EUR 490.2

ART 1000: non-filtered: non-sterile
PIP2374	PK2800	EUR 337.44

ART 1000: non-filtered: non-sterile
PIP2376	PK2800	EUR 300.96

Block. 1.25in/32mm in height. Custom drilled with up to 20 holes
BCM1405-BCU	1 pcs, 1 UNIT	EUR 381.11

Block. 1.25in/32mm in height. Custom drilled with up to 20 holes
BSH100-CU	1 PC	EUR 164.65

Block. 1.25in/32mm in height. Custom drilled with up to 20 holes
BLO1240	EACH	EUR 299.82

San Miguel sea lion viral disease One-Step PCR kit
Oneq-V276-100R	100T	EUR 1932

San Miguel sea lion viral disease One-Step PCR kit
Oneq-V276-150R	150T	EUR 2646

San Miguel sea lion viral disease One-Step PCR kit
Oneq-V276-50R	50T	EUR 1410

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) NO iHNHeLa cell line control (50ug DNA)
DR5000A-Custom-Sce	50ug DNA	EUR 3298.8

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) NO iHNHeLa cell line control (100ug DNA)
DR5000A-Custom-Sce2	100ug DNA	EUR 3610.8

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) NO iHNHeLa cell line control (250ug DNA)
DR5000A-Custom-Sce3	250ug DNA	EUR 4234.8

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) Includes HRHeLa cell line control (50ug DNA)
DR3000-Custom-Sce	50ug DNA	EUR 3798

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) Includes HRHeLa cell line control (250ug DNA)
DR3000-Custom-Sce2	250ug DNA	EUR 4110

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) Includes HRHeLa cell line control (100ug DNA)
DR3000-Custom-Sce3	100ug DNA	EUR 4734

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (50ug DNA)
DR3000A-Custom-Sce	50ug DNA	EUR 3298.8

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (100ug DNA)
DR3000A-Custom-Sce2	100ug DNA	EUR 3610.8

Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (250ug DNA)
DR3000A-Custom-Sce3	250ug DNA	EUR 4234.8

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) Includes iHNHeLa cell line control (50ug DNA)
DR5000-Custom-Sce	50ug DNA	EUR 3798

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) Includes iHNHeLa cell line control (100ug DNA)
DR5000-Custom-Sce2	100ug DNA	EUR 4110

Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.) Includes iHNHeLa cell line control (250ug DNA)
DR5000-Custom-Sce3	250ug DNA	EUR 4734

Equine viral arteritis PCR kit
PCR-V160-48R	50T	EUR 987.6

Equine viral arteritis PCR kit
PCR-V160-96R	100T	EUR 1335.6

Feline viral rhinotracheitis PCR kit
PCR-V170-48D	50T	EUR 543.6

Feline viral rhinotracheitis PCR kit
PCR-V170-96D	100T	EUR 686.4

Recombinant Viral MIP-2 Protein
PROTQ98157	50ug	EUR 380.4
Description: vMIP-2 is a chemokine analog encoded by human herpes virus, and has been shown to have antagonist activity towards several chemokine receptors. Recombinant vMIP-2 is a 7.9 kDa protein consisting of 70 amino acids including the four highly conserved cysteine residues present in CC chemokines.

Anti-Viral Protein U Antibody
STJ503506	100 µg	EUR 618

Lenti-scFv TAC Viral Particle
VP-TAC-CR001	1x10^8 TU	Ask for price
Description: T cell antigen coupler (TAC) Viral Particle packaging

Rapid Viral RNA Extraction Kit
VT4184	50Preps	EUR 184.24

Zenoquick Viral DNA Purification Kit
Z2009-050	50 preps	EUR 408

Zenoquick Viral DNA Purification Kit
Z2009-200	4x50 preps	EUR 1129.2

QPCR Kit RNA Viral Hemor
MOL8912	EACH	EUR 839.04

EpiMag Viral RNA Isolation Kit
P-9108-050	50 samples	EUR 152.9

LAG3 CRISPR/Cas9 Lentivirus (Non-Integrating)
78060	500 µl x 2	EUR 995
Description: Lymphocyte-activation gene 3 (LAG3, CD223) is a cell surface protein that belongs to the immunoglobulin (Ig) superfamily. LAG3 is expressed on activated T-cells, Natural Killer cells, B-cells, and plasmacytoid dendritic cells. Its main ligand is the MHC class II, to which it binds with higher affinity than CD4. It negatively regulates cellular proliferation, activation, and homeostasis of T-cells in a similar fashion as CTLA-4 and PD-1, and has been reported to play a role in T-reg suppressive function. A number of LAG3 antibodies are in preclinical development for the treatment of cancer and autoimmune disorders. LAG3 may be a better immune checkpoint inhibitor target than CTLA-4 or PD-1, because antibodies targeting CTLA-4 or PD-1 only activate effector T-cells while failing to inhibit T-reg activity, whereas an antagonist LAG3 antibody can both activate effector T-cells (by downregulating the LAG3 inhibiting signal) and inhibit induced (i.e. antigen-specific) T-reg suppressive activity. The LAG3 CRISPR Lentiviruses are replication incompetent, HIV-based, VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human LAG3 (GenBank Accession #NM_002286) driven by a U6 promoter._x000D_Note: unlike Human LAG3 CRISPR/Cas9 Lentivirus (Integrating) (BPS Bioscience, #78053), the Human LAG3 CRISPR/Cas9 Lentivirus (Non-Integrating) is made with a mutated Integrase, resulting in only transient expression of the Cas9 and LAG3-targeting sgRNA. While this may minimize potential off-targeting risks due to either prolonged expression or integration of the Cas9, puromycin selection should not be used for more than 48 hours post-transduction, which may lower knockout efficiency.

CTLA4 CRISPR/Cas9 Lentivirus (Non-Integrating)
78061	500 µl x 2	EUR 995
Description: CTLA4 (Cytotoxic T-Lymphocyte Associated Protein), also known as CD152, is a protein receptor that functions as an immune checkpoint. It is expressed by activated T-cells and transmits an inhibitory signal to T-cells. CTLA4 is homologous to the T-cell co-stimulatory protein CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells. CTLA4 binds CD80 and CD86 with greater affinity and avidity than CD28, thus enabling it to out-compete CD28 for its ligands and act as an "off" switch when bound to CD80 or CD86. CTLA4 is an important immunotherapy target for the treatment of cancer and autoimmune diseases._x000D_ The CTLA4 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human CTLA4, GenBank Accession #NM_005214, driven by a U6 promoter._x000D_ Note: Unlike human CTLA4 CRISPR/Cas9 Lentivirus (Integrating) (BPS Bioscience, #78054), the Human CTLA4 CRISPR/Cas9 Lentivirus (Non-Integrating) is made with a mutated Integrase, resulting in only transient expression of the Cas9 and CTLA4 targeting sgRNA. While this may minimize potential off-targeting risks due to either prolonged expression or integration of the Cas9, puromycin selection should not be used for more than 48 hours post-transduction, which may lower knockout efficiency.

TCR CRISPR/Cas9 Lentivirus (Non-Integrating)
78062	500 µl x 2	EUR 995
Description: The T-Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Activation of the TCR results in activation of downstream NFAT signaling. The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains._x000D_ The TCR CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human TRAC (T-Cell Receptor Alpha Constant) and human TRBC1 (T-Cell Receptor Beta Constant 1) regions of the α and β chains._x000D_ The non-integrating lentivirus is made with a mutated integrase, resulting in only transient expression of the Cas9 and sgRNA. Although using the non-integrating lentivirus results in lower knockdown efficiency, the Cas9 isn't permanently expressed, which lowers the risk of off-targeting, and there are no random integrations into the cell's genome. Knockout cell lines can still be generated following cell sorting or limited dilution, because even though the Cas9 and sgRNA expression is transient, the changes in the genomic DNA from the Cas9 nuclease activity and NHEJ repair are permanent._x000D_

CD47 CRISPR/Cas9 Lentivirus (Non-Integrating)
78063	500 µl x 2	EUR 995
Description: CD47 (also known as Rh-associated protein, GP42, Integrin-Associated Protein (IAP), or Neurophilin) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on macrophages. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, generating a "don't eat me"signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance._x000D_The CD47 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human CD47 (NM_198793.2) driven by a U6 promoter._x000D_ Note: unlike human CD47 CRISPR/Cas9 Lentivirus (Integrating) (BPS Bioscience, #78056), the human CD47 CRISPR/Cas9 Lentivirus (Non-Integrating) is made with a mutated Integrase, resulting in only transient expression of the Cas9 and CD47 targeting sgRNA. While this may minimize potential off-targeting risks due to either prolonged expression or integration of the Cas9, puromycin selection should not be used for more than 48 hours post-transduction, which may lower knockout efficiency.

TIGIT CRISPR/Cas9 Lentivirus (Non-Integrating)
78065	500 µl x 2	EUR 995
Description: TIGIT (T-cell immunoreceptor with Ig and ITIM domains; VSTM3; VSIG9) is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells and activated CD4+, CD8+, and regulatory T-cells. Interaction with the Poliovirus Receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits either the Src homology (SH) domain-containing tyrosine phosphatases SHP1 and SHP2, or the Inositol phosphatase SHIP1 and SHIP2, to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-κB and NFAT T-cell receptor (TCR) signaling, which blocks T-cell proliferation and cytokine production. TIGIT also serves as a competitive inhibitor of CD226, a costimulatory receptor for CD155. TIGIT-targeting antibodies which block this T-cell intrinsic inhibitory effect have shown enhanced anti-tumor and anti-viral functions in preclinical studies._x000D_ The TIGIT CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human TIGIT (GenBank Accession #NM_173799) driven by a U6 promoter (Figures 1 and 2)._x000D_Note: unlike human TIGIT CRISPR/Cas9 Lentivirus (Integrating) (BPS Bioscience, #78058), the human TIGIT CRISPR/Cas9 Lentivirus (Non-Integrating) is made with a mutated Integrase, resulting in only transient expression of the Cas9 and TIGIT targeting sgRNA. While this may minimize potential off-targeting risks due to either prolonged expression or integration of the Cas9, puromycin selection should not be used for more than 48 hours post-transduction, which may lower knockout efficiency.

CRBN CRISPR/Cas9 Lentivirus (Non-Integrating)
78518	500 µl x 2	EUR 995
Description: Cereblon (CRBN) forms an E3 ubiquitin ligase complex which is responsible for ubiquitinating proteins that regulate various developmental processes. CRBN also binds to Calcium Activated Potassium Channel subunit alpha-1 (KCNMA1) to regulate ion transport. Moreover, mutations in CRBN may play an underlying role in tumor cells acquiring resistance to immunotherapy.The CRBN CRISPR/Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to transduce into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human CRBN.The non-integrating lentivirus is made with a mutated integrase, resulting in only transient expression of the Cas9 and sgRNA. Although using the non-integrating lentivirus results in lower knockdown efficiency, the Cas9 is not permanently expressed, which lowers the risk of off-targeting, and there are no random integrations into the cell's genome. Knockout cell lines can still be generated following cell sorting or limited dilution, because even though the Cas9 and sgRNA expression is transient, the changes in the genomic DNA from the Cas9 nuclease activity and NHEJ repair are permanent.

TGFBR2 CRISPR/Cas9 Lentivirus (Non-Integrating)
78536	500 µl x 2	EUR 995
Description: Transforming growth factor beta receptor 2 (TGFBR2) encodes the TGF-β receptor protein, a transmembrane protein that forms a heterodimeric complex with other receptor proteins and binds TGF-β. This receptor/ligand complex phosphorylates proteins which regulate cell proliferation, cell cycle arrest, wound healing, and immunosuppression. Mutations in TGFBR2 have been linked with Marfan syndrome and the development of various types of tumors.The TGFBR2 CRISPR/Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles ready to infect most types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human TGFBR2 (Figure 1 and Table 1).The TGFRBR2 CRISPR/Cas9 non-integrating lentivirus is made with a mutated integrase, resulting in only transient expression of Cas9 and sgRNA. Although using the non-integrating lentivirus results in lower knockdown efficiency, the Cas9 protein is not permanently expressed, which lowers the risk of off-targeting, and there are no random integrations into the cell's genome. Despite transient expression of Cas9 and sgRNA, knockout cell lines can still be generated using cell sorting or limiting dilution due to the permanent changes in the genomic DNA from the Cas9 nuclease activity and NHEJ repair.

FCGR2A CRISPR/Cas9 Lentivirus (Non-Integrating)
78538	500 µl x 2	EUR 995
Description: Fc Gamma Receptor 2A (also known as CD32A, Fc-gamma RIIa, FcgRIIa) is a low affinity Fc receptor for immunoglobulin G, encoded by the FCGR2A gene. Fc Gamma Receptor 2A is a cell surface receptor that is expressed on a variety of immune cells such as macrophages and neutrophils. It is involved in phagocytosis and in the clearing of spent immune complexes from the circulation. A polymorphism in FCGR2A has been associated with increased risks of nephritis and lupus.The FCGR2A CRISPR/Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles that are ready to infect most types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human FCGR2A.The non-integrating lentivirus is made with a mutated integrase, resulting in only transient expression of Cas9 and sgRNA. Although using the non-integrating lentivirus results in lower knockdown efficiency, Cas9 is not permanently expressed, which lowers the risk of off-targeting, and there are no random integrations into the cell's genome. Despite transient expression of Cas9 and sgRNA, knockout cell lines can still be generated using cell sorting or limiting dilution due to the permanent changes in the genomic DNA from the Cas9 nuclease activity and NHEJ repair.

NLRP3 CRISPR/Cas9 Lentivirus (Non-Integrating)
78546	500 µl x 2	EUR 795
Description: NLR family Pyrin domain containing 3 (NLRP3) is expressed in macrophages and is a component of inflammasomes. NLRP3 detects uric acid and extracellular ATP in damaged tissue and interacts with a pro-apoptotic protein that recruits caspases. This complex is also an upstream activator of NF-κB signaling and triggers an immune response as part of the innate immune system. Mutations in NLRP3 are known to cause autoinflammatory and neuroinflammatory diseases such as Alzheimer's, Parkinson's, and prion disease. The NLRP3 CRISPR/Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles ready to be transduced into most  types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human NLRP3 (Figure 1 and Table 1), allowing the knockdown of NLRP3 in transduced cellsThe non-integrating lentivirus is made with a mutated integrase, resulting in only transient expression of the Cas9 and sgRNA. Although using the non-integrating lentivirus results in lower knockdown efficiency, the Cas9 protein is not permanently expressed, which lowers the risk of off-targeting, and there are no random integrations into the cell's genome Despite transient expression of Cas9 and sgRNA, knockout cell lines can be generated using cell sorting or limiting dilution due to the permanent changes in the genomic DNA from the Cas9 nuclease activity and NHEJ repair.

All-in-One cDNA Synthesis SuperMix
B24403	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

5X All-In-One RT MasterMix
G485	25 x 10 ul reactions	EUR 79.2

5X All-In-One RT MasterMix
G486	100 x 10 ul reactions	EUR 126

5X All-In-One RT MasterMix
G490	200 x 10 ul reactions	EUR 169.2

5X All-In-One RT MasterMix
G590	1000 x 10 ul reactions	EUR 548.4

Star T940 All-in-one Titrator
START9400	EACH	EUR 5719.38

Mrpl34 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195105	3 x 1.0 ug	EUR 451.2

Olr45 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195205	3 x 1.0 ug	EUR 451.2

Olr1086 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195305	3 x 1.0 ug	EUR 451.2

Olr1380 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195405	3 x 1.0 ug	EUR 451.2

Olr1020 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195505	3 x 1.0 ug	EUR 451.2

Ttc5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195605	3 x 1.0 ug	EUR 451.2

Cd302 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7195705	3 x 1.0 ug	EUR 451.2

Custom CRISPR All-in-one non-viral