DNAfectin Plus Transfection Reagent

DNAfectin Plus Transfection Reagent 

To Order Contact us: lieven@wlsolutions.be

Transfection Reagent (1 mL)

P901 - Ask for price

Transfection Reagent (0.1 mL)

P901S - Ask for price

HighGene transfection reagent

RM09014 1000μl
EUR 324

iMFectin 2000 DNA Transfection Reagent

I7300-101 1ml Ask for price

iMFectin 2000 DNA Transfection Reagent

I7300-105 5x1ml Ask for price

ExFect2000 Transfection Reagent

T202-01 0.5 ml
EUR 272.4

ExFect2000 Transfection Reagent

T202-02 1 ml
EUR 379.2

ExFect2000 Transfection Reagent

T202-03 5 ml
EUR 1262.4

PureFection Transfection Reagent

LV750A-1 1 ml
EUR 303

Transfectamineâ„¢ 5000 Transfection Reagent

60020-05mL 0.5 mL
EUR 222
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent

60021-1mL 1 mL
EUR 334
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent

60022-5mL 5 mL
EUR 846
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

iMFectin Poly DNA Transfection Reagent

I7200-100 100ul
EUR 176.4

iMFectin Poly DNA Transfection Reagent

I7200-101 1ml
EUR 530.4

iMFectin Poly DNA Transfection Reagent

I7200-105 5x1ml
EUR 2247.6

iMFectin Poly DNA Transfection Reagent

I7200-120 20ml
EUR 8144.4

Transfectamineâ„¢ mRNA Transfection Reagent

60030-500ul 500 ul
EUR 203
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent

60031-5ml 5 ml
EUR 989
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Lentifectin (TM) Transfection Reagent

DNAF003 1ml
EUR 334.57

Sapphire Insect Transfection Reagent

ABP-BVD-10003 75ul, 25 transfections Ask for price

GeneGlide? siRNA Transfection Reagent

M1081-1000 each
EUR 534

GeneGlide? siRNA Transfection Reagent

M1081-300 each
EUR 318

GeneGlide? siRNA Transfection Reagent

M1081-500 each
EUR 364.8

Transfectamine™ mRNA Transfection Reagent

60030 500 ul
EUR 203

PureFection™ Transfection reagent

LV750A-5 5 ml
EUR 1287

T-Pro P-Fect Transfection Reagent

JT97-N005M 1.0ml/vial
EUR 266.4

RNAfection, RNA transfection reagent 50 ul

MR750A-1 50 ul
EUR 157

T-Pro Nonliposomal Transfection Reagent I (NTR I)

JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)

JT97-N002M 1.0ml/vial
EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)

JT97-N006M 1.0ml/vial
EUR 266.4

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-520A-KIT 1 kit
EUR 1141

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-550A-KIT 1 kit
EUR 1141

pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-575A-KIT 1 kit
EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-599A-KIT 1 kit
EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-639A-KIT 1 kit
EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-649A-KIT 1 kit
EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-659A-KIT 1 kit
EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-669A-KIT 1 kit
EUR 1162

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-619A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-629A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-589A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-609A-KIT 1 kit
EUR 1141

FBS, Transfection Optimized

F0640-050 500ml
EUR 902.4

293T Transfection Kit (1 mL)

P902 - Ask for price

293T Transfection Kit (0.2 mL)

P902S - Ask for price

293T Transfection Kit (1 mL)

P903 - Ask for price

293T Transfection Kit (0.2 mL)

P903S - Ask for price

Calcium Phosphate Transfection Kit

GR103070 100 preps
EUR 219

Sapphire Baculovirus DNA and Transfection Kit

ABP-BVD-10002 1 kit Ask for price

Protoplast Solution for Transfection

GR103052 10 mL
EUR 119

Exo-Fect Exosome Transfection Kit

EXFT10A-1 10 reactions
EUR 238

Exo-Fect Exosome Transfection Kit

EXFT20A-1 20 reactions
EUR 429

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)

DR5001-HNHela-Sce2 100ug DNA
EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)

DR5001-HNHela-Sce3 250ug DNA
EUR 4110

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)

DR3000-HRHeLa-CPR2 100ug DNA
EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)

DR3000-HRHeLa-CPR3 250ug DNA
EUR 4234.8

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)

DR3000-HRHeLa-Sce2 100ug DNA
EUR 3486

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)

DR3000-HRHeLa-Sce3 250ug DNA
EUR 4110

Exo-Fect siRNA/miRNA Transfection Kit

EXFT200A-1 20 reactions
EUR 412

NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA)

DR5001-HNHela-Sce 50ug DNA
EUR 3174

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA)

DR3000-HRSH-Sce2 100ug DNA
EUR 3735.6

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA)

DR3000-HRSH-Sce3 250ug DNA
EUR 4359.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA)

DR5000-HN3T3-Sce2 100ug DNA
EUR 3486

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA)

DR5000-HN3T3-Sce3 250ug DNA
EUR 4110

Transfection-ready Cas9 SmartNuclease AAVS1 gRNA

CAS520A-1 10 ug
EUR 281

Transfection Collection™ : PDE4D Transient Pack

79286 500 rxns.
EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Phosphate-buffered Sucrose for Transfection

GR103050 1 L
EUR 119

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 119

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)

DR3000-HRHeLa-CPR 50ug DNA
EUR 3298.8

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA)

DR3000-HR3T3-Sce2 100ug DNA
EUR 3486

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (250ug DNA)

DR3000-HR3T3-Sce3 250ug DNA
EUR 4110

HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA)

DR3000-HRHeLa-Sce 50ug DNA
EUR 3174

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA)

DR3000-HRCN1.4-Sce2 100ug DNA
EUR 3735.6

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA)

DR3000-HRCN1.4-Sce3 250ug DNA
EUR 4359.6

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA)

DR3000-HRSH-Sce 50ug DNA
EUR 3423.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA)

DR5000-HN3T3-Sce 50ug DNA
EUR 3174

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA)

DR5000-HNCN1.4-Sce2 100ug DNA
EUR 3486

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA)

DR5000-HNCN1.4-Sce3 250ug DNA
EUR 4110

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA)

DR5000-HNSH-Sce2 100ug DNA
EUR 3610.8

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA)

DR5000-HNSH-Sce3 250ug DNA
EUR 4234.8

100 x DEAE-dextran Stock Solution for Transfection

GR103049 10 x 10 mL
EUR 119

Bacteria RNA Plus Reagent

R402-01 20 ml
EUR 156

Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)

CAS500A-1 20 ug
EUR 387

"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready)

CVD19-420A-1 10 µg
EUR 328

"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)

CVD19-425A-1 20 µg
EUR 468

"S" SARS-CoV-2 Spike Omicron mRNA (10 µg, Transfection-ready)

CVD19-440A-1 10 µg
EUR 383

"S" SARS-CoV-2 Spike Omicron mRNA (20 µg, Transfection-ready)

CVD19-445A-1 20 µg
EUR 553

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA)

DR3000-HR3T3-Sce 50ug DNA
EUR 3174

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 - Ask for price

"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 µg, Transfection-ready)

CVD19-430A-1 10 µg
EUR 360

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 µg, Transfection-ready)

CVD19-435A-1 20 µg
EUR 520

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA)

DR3000-HRCN1.4-Sce 50ug DNA
EUR 3423.6

Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway)

79266 500 rxns.
EUR 715
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway)

79284 500 rxns.
EUR 750
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259).

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA)

DR5000-HNCN1.4-Sce 50ug DNA
EUR 3174

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA)

DR5000-HNSH-Sce 50ug DNA
EUR 3298.8

Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway)

79271 500 rxns.
EUR 750
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version)

CAS504A-1 20 ug
EUR 387

Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway)

79272 500 rxns.
EUR 750
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway)

79268 500 rxns.
EUR 780
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway)

79285 500 rxns.
EUR 715
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,

The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway

79264 500 rxns.
EUR 715
Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway)

79267 500 rxns.
EUR 715
Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway

79265 500 rxns.
EUR 715
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway

79273 500 rxns.
EUR 750
Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker)

CAS530G-1 10 ug
EUR 342

Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker)

CAS531R-1 10 ug
EUR 342

DNAfectin Plus Transfection Reagent