DNAfectin Plus Transfection Reagent
To Order Contact us: lieven@wlsolutions.be
Transfection Reagent (1 mL) |
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| P901 | - | Ask for price |
Transfection Reagent (0.1 mL) |
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| P901S | - | Ask for price |
HighGene transfection reagent |
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| RM09014 | 1000μl | EUR 324 |
iMFectin 2000 DNA Transfection Reagent |
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| I7300-101 | 1ml | Ask for price |
iMFectin 2000 DNA Transfection Reagent |
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| I7300-105 | 5x1ml | Ask for price |
ExFect2000 Transfection Reagent |
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| T202-01 | 0.5 ml | EUR 272.4 |
ExFect2000 Transfection Reagent |
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| T202-02 | 1 ml | EUR 379.2 |
ExFect2000 Transfection Reagent |
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| T202-03 | 5 ml | EUR 1262.4 |
PureFection Transfection Reagent |
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| LV750A-1 | 1 ml | EUR 303 |
Transfectamineâ„¢ 5000 Transfection Reagent |
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| 60020-05mL | 0.5 mL | EUR 222 |
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Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
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Transfectamineâ„¢ 5000 Transfection Reagent |
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| 60021-1mL | 1 mL | EUR 334 |
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Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
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Transfectamineâ„¢ 5000 Transfection Reagent |
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| 60022-5mL | 5 mL | EUR 846 |
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Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
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iMFectin Poly DNA Transfection Reagent |
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| I7200-100 | 100ul | EUR 176.4 |
iMFectin Poly DNA Transfection Reagent |
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| I7200-101 | 1ml | EUR 530.4 |
iMFectin Poly DNA Transfection Reagent |
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| I7200-105 | 5x1ml | EUR 2247.6 |
iMFectin Poly DNA Transfection Reagent |
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| I7200-120 | 20ml | EUR 8144.4 |
Transfectamineâ„¢ mRNA Transfection Reagent |
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| 60030-500ul | 500 ul | EUR 203 |
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Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
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Transfectamineâ„¢ mRNA Transfection Reagent |
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| 60031-5ml | 5 ml | EUR 989 |
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Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
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Lentifectin (TM) Transfection Reagent |
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| DNAF003 | 1ml | EUR 334.57 |
Sapphire Insect Transfection Reagent |
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| ABP-BVD-10003 | 75ul, 25 transfections | Ask for price |
GeneGlide? siRNA Transfection Reagent |
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| M1081-1000 | each | EUR 534 |
GeneGlide? siRNA Transfection Reagent |
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| M1081-300 | each | EUR 318 |
GeneGlide? siRNA Transfection Reagent |
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| M1081-500 | each | EUR 364.8 |
Transfectamine™ mRNA Transfection Reagent |
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| 60030 | 500 ul | EUR 203 |
PureFection™ Transfection reagent |
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| LV750A-5 | 5 ml | EUR 1287 |
T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | 1.0ml/vial | EUR 266.4 |
RNAfection, RNA transfection reagent 50 ul |
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| MR750A-1 | 50 ul | EUR 157 |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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| JT97-N001M | 1.0ml/vial | EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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| JT97-N002M | 1.0ml/vial | EUR 244.8 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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| JT97-N006M | 1.0ml/vial | EUR 266.4 |
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-520A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-550A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-575A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-599A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-639A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-649A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-659A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-669A-KIT | 1 kit | EUR 1162 |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-619A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-629A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-589A-KIT | 1 kit | EUR 1141 |
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-609A-KIT | 1 kit | EUR 1141 |
FBS, Transfection Optimized |
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| F0640-050 | 500ml | EUR 902.4 |
293T Transfection Kit (1 mL) |
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| P902 | - | Ask for price |
293T Transfection Kit (0.2 mL) |
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| P902S | - | Ask for price |
293T Transfection Kit (1 mL) |
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| P903 | - | Ask for price |
293T Transfection Kit (0.2 mL) |
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| P903S | - | Ask for price |
Calcium Phosphate Transfection Kit |
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| GR103070 | 100 preps | EUR 219 |
Sapphire Baculovirus DNA and Transfection Kit |
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| ABP-BVD-10002 | 1 kit | Ask for price |
Protoplast Solution for Transfection |
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| GR103052 | 10 mL | EUR 119 |
Exo-Fect Exosome Transfection Kit |
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| EXFT10A-1 | 10 reactions | EUR 238 |
Exo-Fect Exosome Transfection Kit |
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| EXFT20A-1 | 20 reactions | EUR 429 |
NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) |
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| DR5001-HNHela-Sce2 | 100ug DNA | EUR 3486 |
NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) |
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| DR5001-HNHela-Sce3 | 250ug DNA | EUR 4110 |
HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) |
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| DR3000-HRHeLa-CPR2 | 100ug DNA | EUR 3610.8 |
HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) |
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| DR3000-HRHeLa-CPR3 | 250ug DNA | EUR 4234.8 |
HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA) |
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| DR3000-HRHeLa-Sce2 | 100ug DNA | EUR 3486 |
HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA) |
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| DR3000-HRHeLa-Sce3 | 250ug DNA | EUR 4110 |
Exo-Fect siRNA/miRNA Transfection Kit |
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| EXFT200A-1 | 20 reactions | EUR 412 |
NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA) |
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| DR5001-HNHela-Sce | 50ug DNA | EUR 3174 |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA) |
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| DR3000-HRSH-Sce2 | 100ug DNA | EUR 3735.6 |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA) |
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| DR3000-HRSH-Sce3 | 250ug DNA | EUR 4359.6 |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA) |
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| DR5000-HN3T3-Sce2 | 100ug DNA | EUR 3486 |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA) |
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| DR5000-HN3T3-Sce3 | 250ug DNA | EUR 4110 |
Transfection-ready Cas9 SmartNuclease AAVS1 gRNA |
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| CAS520A-1 | 10 ug | EUR 281 |
Transfection Collection™ : PDE4D Transient Pack |
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| 79286 | 500 rxns. | EUR 845 |
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Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
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Phosphate-buffered Sucrose for Transfection |
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| GR103050 | 1 L | EUR 119 |
Plant Electroporation Buffer for Transfection |
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| GR103051 | 1 L | EUR 119 |
HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA) |
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| DR3000-HRHeLa-CPR | 50ug DNA | EUR 3298.8 |
HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA) |
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| DR3000-HR3T3-Sce2 | 100ug DNA | EUR 3486 |
HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (250ug DNA) |
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| DR3000-HR3T3-Sce3 | 250ug DNA | EUR 4110 |
HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA) |
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| DR3000-HRHeLa-Sce | 50ug DNA | EUR 3174 |
HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA) |
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| DR3000-HRCN1.4-Sce2 | 100ug DNA | EUR 3735.6 |
HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA) |
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| DR3000-HRCN1.4-Sce3 | 250ug DNA | EUR 4359.6 |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA) |
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| DR3000-HRSH-Sce | 50ug DNA | EUR 3423.6 |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA) |
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| DR5000-HN3T3-Sce | 50ug DNA | EUR 3174 |
NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA) |
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| DR5000-HNCN1.4-Sce2 | 100ug DNA | EUR 3486 |
NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA) |
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| DR5000-HNCN1.4-Sce3 | 250ug DNA | EUR 4110 |
NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA) |
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| DR5000-HNSH-Sce2 | 100ug DNA | EUR 3610.8 |
NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA) |
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| DR5000-HNSH-Sce3 | 250ug DNA | EUR 4234.8 |
100 x DEAE-dextran Stock Solution for Transfection |
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| GR103049 | 10 x 10 mL | EUR 119 |
Bacteria RNA Plus Reagent |
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| R402-01 | 20 ml | EUR 156 |
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) |
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| CAS500A-1 | 20 ug | EUR 387 |
"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready) |
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| CVD19-420A-1 | 10 µg | EUR 328 |
"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready) |
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| CVD19-425A-1 | 20 µg | EUR 468 |
"S" SARS-CoV-2 Spike Omicron mRNA (10 µg, Transfection-ready) |
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| CVD19-440A-1 | 10 µg | EUR 383 |
"S" SARS-CoV-2 Spike Omicron mRNA (20 µg, Transfection-ready) |
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| CVD19-445A-1 | 20 µg | EUR 553 |
HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA) |
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| DR3000-HR3T3-Sce | 50ug DNA | EUR 3174 |
ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) |
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| P402 | - | Ask for price |
"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 µg, Transfection-ready) |
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| CVD19-430A-1 | 10 µg | EUR 360 |
"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 µg, Transfection-ready) |
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| CVD19-435A-1 | 20 µg | EUR 520 |
HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA) |
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| DR3000-HRCN1.4-Sce | 50ug DNA | EUR 3423.6 |
Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway) |
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| 79266 | 500 rxns. | EUR 715 |
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Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
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Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway) |
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| 79284 | 500 rxns. | EUR 750 |
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Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259). |
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NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA) |
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| DR5000-HNCN1.4-Sce | 50ug DNA | EUR 3174 |
NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA) |
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| DR5000-HNSH-Sce | 50ug DNA | EUR 3298.8 |
Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) |
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| 79271 | 500 rxns. | EUR 750 |
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Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version) |
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| CAS504A-1 | 20 ug | EUR 387 |
Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) |
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| 79272 | 500 rxns. | EUR 750 |
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Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway) |
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| 79268 | 500 rxns. | EUR 780 |
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Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway) |
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| 79285 | 500 rxns. | EUR 715 |
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Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth, |
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The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway |
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| 79264 | 500 rxns. | EUR 715 |
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Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway) |
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| 79267 | 500 rxns. | EUR 715 |
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Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
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The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway |
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| 79265 | 500 rxns. | EUR 715 |
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Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway |
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| 79273 | 500 rxns. | EUR 750 |
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Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker) |
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| CAS530G-1 | 10 ug | EUR 342 |
Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker) |
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| CAS531R-1 | 10 ug | EUR 342 |