EnzyFluo Bile Acid Asasy kit
To Order Contact us: lieven@wlsolutions.be
EnzyFluo™ Isocitrate Assay Kit |
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EFIC-100 | 100 | EUR 399 |
Description: For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585 nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.6 µM. |
EnzyFluo™ NAD/NADH Assay Kit |
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EFND-100 | 100 | EUR 459 |
Description: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.02 µM and linearity up to 1 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: F530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM. |
EnzyFluo™ Acetaldehyde Assay Kit |
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EFAC-100 | 100 | EUR 319 |
Description: For quantitatve determination of acetaldehyde and evaluations of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (50µL sample): 0.5 µM to 60 µM acetaldehyde in 96-well plate assay. High-throughput. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. Convenient. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 µM. |
EnzyFluo™ NADP/NADPH Assay Kit |
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EF2NP-100 | 100 | EUR 479 |
Description: For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). Key Features: Sensitive and accurate. Detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 µM. |
EnzyFluo™ D-Lactate Assay Kit |
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EFDLC-100 | 100 | EUR 439 |
Description: For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM. |
EnzyFluo™ L-Lactate Assay Kit |
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EFLLC-100 | 100 | EUR 439 |
Description: For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM. |
EnzyFluo™ Glucose Uptake Assay Kit |
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EFGU-100 | 100 | EUR 669 |
Description: For quantitative determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport. Key Features: Safe. No radioactive material is used. Sensitive and Accurate. Detection limit of 0.1 µM and linearity up to 5 µM 2-DG6P. Simple and Convenient. Can be automated as a medium throughput assay for glucose transport in cells. Method: FL530/585nm. Samples: Cell culture. Species: Mammalian. Procedure: Assay takes Approximately 2 hrs. Kit size: 100 tests. Detection limit: 0.1 µM. |
EnzyFluo™ ERK Phosphorylation Assay Kit |
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EERK-100 | 100 | EUR 569 |
Description: For quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-based ELISA (FL360/450nm, 530/585nm). Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ NFkB Phosphorylation Assay Kit |
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ENFKB-100 | 100 | EUR 559 |
Description: For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ AMPK Phosphorylation Assay Kit |
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EAMPK-100 | 100 | EUR 559 |
Description: For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Custom Cell-Based ELISA Kit |
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EFC1M-100 | 100 | EUR 359 |
Description: A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary mouse antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Custom Cell-Based ELISA Kit |
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EFC2R-100 | 100 | EUR 359 |
Description: A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary rabbit antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Farnesyltransferase Activity Assay Kit |
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EFTS-400 | 400 | EUR 359 |
EnzyFluo™ Farnesyltransferase Inhibitor Screening Kit |
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EIFT-400 | 400 | EUR 369 |
EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit |
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ESHP2-100 | 100 | EUR 509 |
Description: For determination of SHP2(Y542) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse GSK3B(S9) Phosphorylation ELISA Kit |
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EGSK3B-100 | 100 | EUR 509 |
Description: For determination of GSK3B(S9) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit |
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EAKT1-100 | 100 | EUR 509 |
Description: For determination of AKT1(S473) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit |
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ELKB1-100 | 100 | EUR 509 |
Description: For determination of LKB1(S431) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, and rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit |
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E4EBP-100 | 100 | EUR 509 |
Description: For determination of 4EBP1(S65) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human (S65), mouse/rat (S64). Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit |
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EGSK3A-100 | 100 | EUR 509 |
Description: For determination of GSK3A(S21) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat, mouse, and canine. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit |
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ERBT-100 | 100 | EUR 509 |
Description: For determination of RB(T821/826) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluo™ Human/Mouse RPS6(S235/236) Phosphorylation ELISA |
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ERPS6-100 | 100 | EUR 509 |
Description: For determination of RPS6(S235/236) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests. |
EnzyFluoTM Myeloperoxidase Assay Kit |
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EMPO-100 | 100 | EUR 429 |
Description: For quantitative determination of myeloperoxidase enzyme activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.0025 to 2 U/L for 10 min reaction at 25°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Cell lysates, tissues, etc. Species: All. Procedure: Assay takes approximately 30 min. Kit size: 100 tests. Detection limit: 0.0025 U/L. |
Total Bile Acid Assay Kit |
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STA-631 | 100 assays | EUR 574.8 |
Description: While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also required for proper uptake of nutrients in the small intestine. The Total Bile Acid Assay Kit provides a convenient 96-well plate-based method to measure the total bile acid content in a variety of sample types. |
Bile acid receptor |
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AP86586 | 1mg | EUR 2640 |
Bile acid receptor |
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AP85793 | 1mg | EUR 2640 |
Bile acid receptor |
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AP86348 | 1mg | EUR 2640 |
Bile acid receptor |
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AP89445 | 1mg | EUR 2640 |
Total Bile Acid Microplate Assay Kit |
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DLSM0153 | 100 Assays | EUR 455 |
Description: Detection and Quantification of Bile Acid Content. |
Total Bile Acid Assay Kit (Fluorometric) |
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MET-5005 | 100 assays | EUR 567.6 |
Description: Total Bile Acid Assay Kit is a colorimetric 96-well plate-based method to measure total bile acid content in a variety of sample types. The assay uses an enzyme driven reaction in which bile acids are incubated in the presence of thio-NAD+. 100 assays. |
Rat Total Bile Acid (TBA) ELISA Kit |
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abx052583-96tests | 96 tests | EUR 943.2 |
Rat TBA(Total Bile Acid) ELISA Kit |
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ELK8664-48T | 48T | Ask for price |
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TBA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TBA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Rat TBA(Total Bile Acid) ELISA Kit |
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ELK8664-96T | 96T | Ask for price |
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TBA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TBA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Rat total bile acid(TBA) ELISA Kit |
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NSL1024r | 96 Tests | EUR 498 |
Bile acid receptor Antibody |
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20-abx109578 |
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